FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1975-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1975-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036388
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC11430.11028688097507883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5100.031.2759252
GTGCGAA1402.7459215E-531.0728364
AGCGAAA5150.030.979751
CTAGTAC950.004726328430.5276953
GCTATAT2151.8055289E-830.3575341
TATACTG5200.027.8845335
CGAAAGC5900.027.0351243
GTAGGCT2954.820322E-1027.033826
TCGCCAT1100.00967188426.363558145
TTAGGTA3501.2914825E-1024.8582694
CCTATAC1500.001493916124.1677593
GTAGTAG2202.2239721E-523.0747931
GTATTAG2003.0971383E-421.7562331
CTACTGT7350.021.7068981
TAGGTAG5051.8189894E-1221.5345885
GTATATA4409.822543E-1121.4265941
GTGATCG1700.00309292321.3234679
ATATAAC2755.2089254E-621.0918643
GTAATAT2806.0755337E-620.7202221
GTATTAT10700.020.3329281
Produced by FastQC (version 0.11.5)