FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1975-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1975-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915040
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12870.14064958908900158No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC12830.1402124497289736No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT12020.13136037768840708No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC10500.11474908200734395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCCG450.00963900748.3331689
CGACCCG657.3363783E-444.61523145
GTTACGT855.3291988E-542.663231
AGCGAAA2950.041.795511
TTAGGTA2900.034.999884
GTATTAT8750.034.81321
CTATGCG2302.7284841E-1134.6737984
GCGAAAG3650.033.7688522
GTAGAAA4050.032.2344441
CGGAGTA900.003628132232.222114
CGAAAGC3850.032.0146263
TTACGTG1153.1609403E-431.5233572
TATACTG2807.2759576E-1231.0713235
CTATTAC1453.481051E-530.011381
GTTGACA7300.029.805821
GCGTTAA1255.1536056E-428.9998999
ACTGGTT3550.028.5914545
GTAATAT2903.929017E-1027.5104311
CCTATGC3503.6379788E-1226.9299533
TTGATTA4050.026.85176145
Produced by FastQC (version 0.11.5)