FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1974-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1974-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200618
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1801.2885903E-732.2341271
TAGTCGG1851.6441663E-731.3511818
GTTGACA10700.024.4015371
GTATAAG3352.1027518E-923.8147681
TACGGTG2151.86622E-523.6045235
AGCGAAA4950.023.4430031
ATGTTGT19650.023.2441486
GGTAGTC1600.002175471322.6561265
CCGACTA3253.990135E-822.3084984
TTAGGTA3955.8935257E-1022.0261144
TAGCATG15600.021.847382
GCGAAAG5750.021.4391292
GCATGTT16200.021.0347144
ACATGGT12850.020.8753725
TAGGGTG5251.8189894E-1220.7141725
GTATATA6450.020.2400341
GGTTATA3601.1719749E-720.146331
TGTTGTT23850.020.0627827
GCTACTA3651.3608042E-719.8629049
TTGTTTA22500.019.6554479
Produced by FastQC (version 0.11.5)