FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1974-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1974-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016737
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG19960.19631428776566606No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC14220.13985917695529917No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA12950.12736823780387652No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA11670.11477894480086788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1152.4774636E-950.4494671
GTTAACG450.00962810448.3474041
GTTGACA11400.033.7159541
TAGTCGG1301.6548549E-533.46148
TATACTG3950.033.037845
ACATGGT12050.031.8878365
GTATTAT9150.030.9106351
GTAATAT3300.030.766531
CATGTTG14450.028.59855
TGACATG15250.026.150013
GCATGTT17250.026.0591494
TAGCATG17350.025.9114992
GGTCCTA19950.025.8018999
TTACGTG2259.096366E-725.7814772
TTAGGTA3201.2405508E-924.9229984
GTAGCAT22300.024.7157121
ATACTGT6550.024.3510486
TGGTCCT21550.024.222648
GATTACC1801.5188305E-424.1701352
AGCATGT18600.024.1689473
Produced by FastQC (version 0.11.5)