FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1974-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1974-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962425
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT36900.38340649920773046No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA26570.27607346026963137No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG14980.15564849209029277No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA13100.1361145024287607No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA12940.13445203522352392No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA12730.13227004701665065No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG11780.12239914798555733No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG11770.12229524378523002No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC11410.11855469257344728No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG10860.11283996155544587No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC9930.10317687092500714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT100.007088717145.014392
CGAATTA350.003566528462.142577
GTAGAAA11350.060.0500142
CTTACGC450.00963722648.335623
AGCGAAA4550.043.0374261
TAGAAAC17050.040.8230483
ACAAGGC13550.039.593918
AGTAGAA17700.038.516651
GCGAAAG5450.035.9209942
AAGGTCG4000.034.437349
CGAAAGC5800.033.7515953
GTTCGTT1301.6547694E-533.461384145
CAAGGCA15850.032.93369
AACAAGG22800.031.4801187
GGTACTG3700.029.3932845
GACAGTA5250.028.9998657
GTCGTTT3600.028.1943138
TATACTG17700.027.8544245
GGTCGTT3700.027.4323067
TATTCAA7750.027.1303185
Produced by FastQC (version 0.11.5)