FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1973_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1973_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT21.9607843137254901No Hit
GGCCCTGGCCCCCTCCCCACAGTGTTCTATTGATGATGTGGTGCAGTTGC10.9803921568627451No Hit
GTGTTCACGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGATT10.9803921568627451No Hit
CCCAATTGGTTGTTCAGTAGAATGTTGTTTAGTCTCTGTGTTTATGAATT10.9803921568627451No Hit
ATATAAAGGGGAGCCTACAGAGAGCACTACAGTGAAAGTTTTCTACACCC10.9803921568627451No Hit
AGCCCGAGGCGCTCCCCGGGCCGCGAGGGGGCGAGCAGGCCGCAGACCAG10.9803921568627451No Hit
GGTCGGGGTGGCAGTGATTTGGTAGCGGCGCTATCAAATTATTTTTCTGC10.9803921568627451No Hit
ATGATAAGGCCGACCTCACCAGGAGGGTGTGCAATCTGTCTCGCTCTCAT10.9803921568627451No Hit
ATCTGAGACTCTGACCTCACGGCCAATGGGGCTCCCCCACCACCCTGCAC10.9803921568627451No Hit
GGCCTGCCCCCGCCTCCCGCAGCCCTTCTGTGCGACCTGCGGGTCCTGCC10.9803921568627451No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA10.9803921568627451No Hit
ACCATGGCCTCCATCATGCTGGATATTCCAACTGGCCTCCGATATGAGCT10.9803921568627451No Hit
CAGATGGACCGCTTAGGACCCTATGGCTCATGTCTCCTAACATCCCTCTT10.9803921568627451No Hit
TGGAAGCCCTGAGACCAGGCAAGGCAAGGGAAGTAGGCAGCGATCCGACA10.9803921568627451No Hit
ACGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATCGAACAG10.9803921568627451No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA10.9803921568627451No Hit
GCTCCATTGGGCTGAAAATAAGAAGATCAGCTGTTAGTTCTGCAGCCATG10.9803921568627451No Hit
GCCATTATCAATTCTATCAACTTCCCAGTTCTGTACAAATTAGGAAATTT10.9803921568627451No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA10.9803921568627451No Hit
AACATGGACAGGGCTGTTAAACTATACAAGAAGCTGAAGAGGGAAATGCC10.9803921568627451No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA10.9803921568627451No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG10.9803921568627451No Hit
GTCAAAGCATCTCTTGTCGGCCTGTACCCAGCAAGTAAGAAAATAACATG10.9803921568627451No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG10.9803921568627451No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA10.9803921568627451No Hit
CCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAG10.9803921568627451No Hit
AACCAAAATAGGAACACAGAAATACTTTGAGGTAAATGAAAAATTAATAC10.9803921568627451No Hit
ACTCAGACCACACAGCACAGGGCAGAGAGAAGACGGGCAGAAAAGCCCAG10.9803921568627451No Hit
ACAAGGGAGTGTCCTTCACAAAAAGGAGGCCATGCCCTCATTCCGCTGAA10.9803921568627451No Hit
GTCTGCGCCTTATCACGTGGACTCTCAGAACGTCCAGGCTGACTCTTCAT10.9803921568627451No Hit
TGGCCTCCATCATGCTGGAAATTCCAACTGGCATCCGATATTAACTGATA10.9803921568627451No Hit
GACTAGCTCAGGCCAGCAGCAGTAGCATCCGCAAACCCCACTCTGATATA10.9803921568627451No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC10.9803921568627451No Hit
TCAAGGGGGTTCCCACCTCTAGAGTTTCACAACATATTATCTCAGGAACT10.9803921568627451No Hit
ACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGC10.9803921568627451No Hit
AGCAGGGGGAGTGGCAAGCAGAGGAAGAGGGAGAAGCAGGCTCCACGGTG10.9803921568627451No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTTCAATATGCA10.9803921568627451No Hit
GAGAAGGAAGAAAATGAATACAGAAGTTAAAAACATTTCCTACATGACTT10.9803921568627451No Hit
GGTCTGTAGGCTCCGATGGCTCATGGGGCGGAGGAAGGCTGGGTGTGGGC10.9803921568627451No Hit
GGCAAGCGTCACCCGCCTCCTGTGCGAAGAGACGGAAGCGGGGCTGCCAA10.9803921568627451No Hit
GAACTGAAGGCAGCCACTTAACAACTGAGCTACCCAGGAGCCCCTACAAT10.9803921568627451No Hit
TTTGGAGTGTTTCCCTAAGGATTTGCCTCTGGTTGCATTCTAGTCTGATT10.9803921568627451No Hit
AGTAACGGGTTGTCTGAGGCTGATGTTTCCATTGTGATCTTCTGTCTGGA10.9803921568627451No Hit
CACAAAGTGCTGGCCTGGATAATGATCATCTCAATGGACACAACTCCATG10.9803921568627451No Hit
GTCTTTGCACACGTGGTTCCTTCCCCTTCGATCTACTTGTCCCCTTCATC10.9803921568627451No Hit
ATTCTCCCCCAGCCACACACCAGATCACAAAGGAAACAACACCCTCAGAC10.9803921568627451No Hit
GTGTTGCCAGGATTCAAGGAGCACCTTGAATTCAAGGAGCACCTGGCACC10.9803921568627451No Hit
CTCTTGCTGCTCTCCAGGCTGTGGATATGTGGGGCTGACTGCGAGCATGG10.9803921568627451No Hit
GACTAAGGCCAAGGGCTGATCCCACGAGGCAGCTCCGGCCCATGGGCTTG10.9803921568627451No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACT10.9803921568627451No Hit
AGTAATCAATGACCTGATATGTTATACAGATATCGTGAAGCATGGCAAAT10.9803921568627451No Hit
AACCATTTGAATGGATGTCAATCAGACTTTACTTTACTTAAAAGTGCCAG10.9803921568627451No Hit
TTCAGCTGTAAAAGCTCTAAGAAGTATCAGCGCATCAAGTAGATTAAAAA10.9803921568627451No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT10.9803921568627451No Hit
ATCTCAGGTTGAACAAGGCAATGCCATGCCTTCTTGCTTCAGCCAGCTCT10.9803921568627451No Hit
AGTAGATCCAGACTCAGTTCTTGTGTAGTCTCTTGTGGATCTACATGCTT10.9803921568627451No Hit
TGCACACACTGCATTCCAGAAGCCCCCGCGCCCTCCTTTGGGTCCCTTCA10.9803921568627451No Hit
TGATTACACAGACGCTCCCTTCACATAGCTTGCAGTCTGGTGGGAAGAAG10.9803921568627451No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG10.9803921568627451No Hit
AGTAGAAACAGGGCATTTTTTCATGAAGGACCAGCCAAATTCACTTCCCT10.9803921568627451No Hit
TGCCGATACTCTTCCCTCATAGACTCAGGCACTCCTTCCGTAGAAGGCCC10.9803921568627451No Hit
GAATTGAATCACGGAGGGAGAGACCATTCGGCCTTCTAAACCTAGCGTTT10.9803921568627451No Hit
GGTACTGATCCAAAATGGAAGACTTTGTGCGACAGCGCTTTAATCCAATG10.9803921568627451No Hit
GTAAACCCCCCAGTGACACTGATGGGGCTGGTTTACAGACTACAATCTGA10.9803921568627451No Hit
GGGGTGGGGCGGGCGCCGGAGGGGCAGTCCCGGCGCGGCGCGCGGGGGAG10.9803921568627451No Hit
TCCGAGTTCTGTTGGTGGGTGTCTGGCGGTCGGTGAGTGTGCTAGCGGCC10.9803921568627451No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT10.9803921568627451No Hit
GCCGGCTAGAGATCACAAGAAGGTGCGGAAGGAAAGGGCCGGTAAGTGTA10.9803921568627451No Hit
ATGCACACACTTAGAAGTCTGCTTACTAGCAAAATCTGTGTTCAACAGCC10.9803921568627451No Hit
GAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATAGGGCAAGGAG10.9803921568627451No Hit
GACTATAGTAAGTTAGCTCAGAATGCCCTAACAAAGTACCGGGACTGGCT10.9803921568627451No Hit
GGATATTGTGGGCCAGGTTGAGCTGCATGAGGTTCTTGAGGCCCTGGAAG10.9803921568627451No Hit
TCGCTATGGGGTATTTTATTATTATGTTCATCCAATGTAGGGCAGAAGCA10.9803921568627451No Hit
GGAAAAAGACAAAGAGTCATCTAAAAATGTCACTATGACGGGCCGACTGG10.9803921568627451No Hit
CTCATAGACTCAGGCGATCCTTACGTAGACGGACCTCTTTTCCAACCGTA10.9803921568627451No Hit
TCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATTAATATAGGCTGT10.9803921568627451No Hit
GCGGGGGGAAGTTTTTATAAAAATTTCCCATTATGTGGGGGGGGGGGGCA10.9803921568627451No Hit
ATGTAAGGCCACAAGACAAGTCATTAAATTCCAGAAGCCTATAAGTTTCC10.9803921568627451No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT10.9803921568627451No Hit
CAATAGGGGTGTGAGAGAGGCCGGGGTGCGGCCAGCCCCGCCTCTGGGGG10.9803921568627451No Hit
GCTATTATTAGCACCATGATTAGGCTGAATTTAGACTAGCATTTCTGTTT10.9803921568627451No Hit
CTCCTGGGATGTACCCTCTGCAGCCTTCCAGTAAAATCCCCATCTCTGAT10.9803921568627451No Hit
GTAGTTTGGAGGCCATTTCCAAGGAGTCTGCCCCCCCGTTACAGAGCGGG10.9803921568627451No Hit
GCTTGTTGGGGTGTAATTGCTCATAGTACATTCTTAAGAGCCTTTGCATA10.9803921568627451No Hit
CACCAACAAGAATAACAGATCAGCAAAGAGCAGGTACTATGAAAAGAACA10.9803921568627451No Hit
TTTTTCGGCTGTTCTTGATTTCAACTCTCTCATGGTCAGTTATGGTCCAT10.9803921568627451No Hit
TCAGAAATGAGAGAGATGTTCCCTGAAACCATTGATATGCAAAGGCTCTT10.9803921568627451No Hit
GGGCTATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTT10.9803921568627451No Hit
CACCTAGCTTAGCGCTCTCATGGTCTCACTTAATCCTCCCCCAAACCCAT10.9803921568627451No Hit
GTATGCTAGAGTCCAGTGTACGGTTACTTACACCACATAAGAATAACTTA10.9803921568627451No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG10.9803921568627451No Hit
TCAAAAACCCAAGCTCCTGGGAGAGAGCCCTGCACCAGGCTCCACACTCA10.9803921568627451No Hit
GGGTTTTGGCGCGGGTGTGCTGGTGTGGAGAGGGCTCGCGTGGGCCCGCC10.9803921568627451No Hit
GTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTG10.9803921568627451No Hit
GATTAATGAAATTCAAGTAAACTCTACAGTTTAGTTAATTATAAAGTGCC10.9803921568627451No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG10.9803921568627451No Hit
GCATTAAGAAAAACCCAGGGATCATTAAACACACAATACTCAATTCATCC10.9803921568627451No Hit
GATTCGTAGAATTCACTGGTGAAACCATGGGGTCCTGGACTTTTGCTTGT10.9803921568627451No Hit
AAAGAGTTCTAAGAAGCAAGTTTATAGTGATACAGGCCTAACAAGAAACA10.9803921568627451No Hit
GGCTGCGGCTGTTGCCCTGTGCCGATCTCGGTGGTATCCGAATCCTACAC10.9803921568627451No Hit
GTGGTTGCGCAATCATTTTCATAATTGGGCAGTATCTCCTAGTTTATAAA10.9803921568627451No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers