FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1973-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1973-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688927
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACT28260.4102031129568155No Hit
GTAGTATGTAGGCCATTTCCCAGTAGTGTGACCCCACTTTCCATAGCTGG21780.3161438004316858No Hit
TTCCATAGCTGGAGAATGTATTCATCACTATTCCCATGTCATGGGGTCGG16140.2342773617524063No Hit
GATCCATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATTCAGTAACGTT16140.2342773617524063No Hit
ACCCAGGACTAACACACGGGTGGGTCCTGCGATCAGGGCCCTGCGCATGG14670.21293983252216853No Hit
GGTAGTATGTAGGCCATTTCCCAGTAGTGTGACCCCACTTTCCATAGCTG14410.2091658477603578No Hit
GTCATGGGGTCGGTGAGCATGACCCAGGACTAACACACGGGTGGGTCCTG13730.19929542607562195No Hit
CTCTGGGAAAGATCCATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATT13350.19377960219297546No Hit
CAGTAACGTTGGGCAAGTTGTTCTGTCCTCTTGCCACAGCTTCCATGTGG13300.19305383589262723No Hit
GTCATGAAGATTCCTGGCTTGCAAGTCCAAGGCCAGGTAGTATGTAGGCC13250.19232806959227902No Hit
TCACTATTCCCATGTCATGGGGTCGGTGAGCATGACCCAGGACTAACACA12050.17490967838392169No Hit
GAAAAGAGGAGAGAGGAATGGAGTCAGCCCGTGAGGTGGGGGGATTGCAC11550.16765201538043942No Hit
GGAATGGAGTCAGCCCGTGAGGTGGGGGGATTGCACTCTGGGAAAGATCC11310.16416833713876797No Hit
GGCTTAACCACAGGGAGACACAGAGATCTGTCATGAAGATTCCTGGCTTG11300.16402318387869833No Hit
TTTCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCA11110.16126527193737505No Hit
GTGAAGGAGCGGAACAAAAATTGACACGGGGACAGGAGGCAGCTGAGTCA11060.16053950563702685No Hit
ATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATTCAGTAACGTTGGGCA10920.15850735999605184No Hit
GAGTCAGCCCGTGAGGTGGGGGGATTGCACTCTGGGAAAGATCCATAGAA10680.15502368175438036No Hit
ATGTAGGCCATTTCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAAT10370.1505239306922214No Hit
ATTCCCATGTCATGGGGTCGGTGAGCATGACCCAGGACTAACACACGGGT10240.148636938311316No Hit
GTATTTGTTCAGATTCATTCAATTGAAAATGATGGAAACCTGAGTCAACA9310.13513768512483906No Hit
GCTTCCATGTGGGTCAGTGAAGGAGCGGAACAAAAATTGACACGGGGACA9150.13281523296372474No Hit
ATTCATCACTATTCCCATGTCATGGGGTCGGTGAGCATGACCCAGGACTA8700.12628333626059074No Hit
ATCCAGTTCCATTTGTGCCCCCAGCTCATTCAGTAACGTTGGGCAAGTTG8610.12497695691996394No Hit
GTATTCATCACTATTCCCATGTCATGGGGTCGGTGAGCATGACCCAGGAC8420.12221904497864071No Hit
GTTCTGTCCTCTTGCCACAGCTTCCATGTGGGTCAGTGAAGGAGCGGAAC8260.11989659281752638No Hit
GTGGAAGAGGGAAAGGGGAGAGAAAAGAGGAGAGAGGAATGGAGTCAGCC7990.11597745479564599No Hit
CTGGGAAAGATCCATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATTCA7970.11568714827550669No Hit
GAATGGAGTCAGCCCGTGAGGTGGGGGGATTGCACTCTGGGAAAGATCCA7850.11394530915467095No Hit
CTATTCCCATGTCATGGGGTCGGTGAGCATGACCCAGGACTAACACACGG7730.11220347003383523No Hit
CTCCTGGAATCCTCCTCCTGTGAGGCCTCGTGTGGTTTGGGGCTGGCGCC7600.11031647765292986No Hit
AAAGCAGGCTGGCCCTGGCTTAACCACAGGGAGACACAGAGATCTGTCAT7570.10988101787272092No Hit
GGATTGCACTCTGGGAAAGATCCATAGAATCCAGTTCCATTTGTGCCCCC7520.1091552515723727No Hit
GTGTGTATGTGTATGTGTGTGTACGCACGCCATTTCTTCTTTATCTCCTC7100.1030588146494476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2650.093.030651
TAATATC350.003565268862.1462174
GAGCGAA954.5474735E-1061.0603681
GCGAAAG4400.056.0257572
ATGCGGA400.00605053254.3739936
GTCTAAT450.00963305348.339451
ACGGGAT604.942686E-448.3359453
TGATGCG450.00963581648.3359454
TATACGG752.5500289E-548.332445
CTCCGCG450.0096385848.3324369
ACTGTCG450.0096385848.332436145
CGAAAGC5450.045.23183
GTCGGTT700.001056508241.427803145
TAGGATT750.001482671438.668764
CTACGGG800.0020349136.2545931
AGTAGAA2600.036.254591
AAAGCAG13150.034.738241
TACGGGA850.002740669334.119492
ATAGACC2206.220944E-1032.9563263
GGATCCG1102.435631E-432.9539349
Produced by FastQC (version 0.11.5)