FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1973-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1973-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844797
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11530.1364824922436988No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC10410.12322486940649648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1300.066.922759
AGCGAAA3100.039.7696381
GTTACGT900.003622746332.23161
GCTACTA1652.3859411E-630.7574279
CTACTGA9150.029.3167956
TAGCATG10850.027.4026662
GCGAAAG4250.027.300452
GTATTAT8650.026.8286191
TACTGAA10500.026.2379677
GTAGCAT13000.025.6613161
GCATGTT11950.024.8758284
CATTCTA9500.024.4267182
CGAAAGC4850.023.920243
GTTGACA6700.023.81291
CATGTTG11500.023.3259745
TTAGAGT5300.023.2559814
AGTCTAT2552.699424E-622.7517191
TAGAGTG4505.456968E-1222.5554455
TTGTTTA15650.022.2363139
ATAGGAC2953.868663E-722.1211553
Produced by FastQC (version 0.11.5)