FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1973-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1973-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142996
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1502.6004273E-838.666129
TATACTG5950.032.904455
GTAATAT3550.032.698481
TATAACG1658.410397E-526.3678785
GCGAAAG4800.025.683462
TAGCATG14250.025.4449142
AGCGAAA4650.024.9633561
GTTACGT2051.28363135E-524.7730871
GTAGCAT16000.024.4855421
ACGTCCG1801.5206172E-424.1663259
GTATTAT12950.024.089881
GCATGTT15500.023.859724
AACCGCG1253.6467085E-523.199677
ATAACGT1902.1929962E-422.8954146
CGAAAGC5550.022.211753
CGGACGC1650.002601298721.969385145
TAACGTC2053.6672349E-421.2192127
GTATAAG2404.3405715E-521.1603431
ACACGAC2104.3150657E-420.713991145
CTACGAG1750.003659346420.7139919
Produced by FastQC (version 0.11.5)