FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1972_lungUR_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1972_lungUR_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047346
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG400.006051027754.37486145
GCTATAT3000.050.766831
AGCGAAA5100.048.3493651
TACGCTA450.00963936348.3332067
GCGAAAG5300.046.5115282
GTTCGTA657.336838E-444.61527145
CTATATA4600.044.1324272
TATATAC6100.042.7867743
CGAAAGC5950.041.4284633
ATATACG1953.6379788E-1240.8973274
GTCGTAG1157.126793E-637.838631
GTATAAG850.002737589234.1289631
CAAGTCG850.002742079334.1175589
TATACGG1301.6548856E-533.4614525
TATACTG4300.032.0347985
GTATTGA1204.0502983E-430.2183531
TCGTAGG1453.4894758E-530.0013542
GAGTCGT1453.4906134E-529.9999226
ATATAGC2451.9717845E-929.5917593
CGACGGC1000.00607466528.999922145
Produced by FastQC (version 0.11.5)