FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1972-lungUR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1972-lungUR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880249
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG10420.1183755959961329No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC10210.11598990740120124No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG8900.10110775473757994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTA1751.0085023E-733.1445964
ATATAAC1801.290864E-732.2257462
AACTTCG1153.1618745E-431.5216057
ATATTAG2101.4395482E-831.0712936
GATTACC950.00472446530.5296542
TATACTG2701.72804E-1029.5385885
CGACCCG1255.153475E-428.999874145
GATATAC1555.4955883E-528.0675853
CCTAGCG1050.00770384927.6220652
ATATATC2756.7557266E-926.3665163
GTAGGCT3057.1122486E-1026.1474276
CTATTAA2507.820199E-826.1028542
TCATAAG5850.026.0284863
TATTGGG3108.56744E-1025.7286192
GTATTAT7950.023.7173271
TATAACC2151.8638302E-523.6072313
CATAAGA4950.023.4355754
ACATGGT7500.023.2012185
ACAACGC1902.192943E-422.8946389
TAGTGTA1902.192943E-422.8946389
Produced by FastQC (version 0.11.5)