FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1972-lungUL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1972-lungUL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539701
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG6650.12321637351051787No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG6430.11914004235678644No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT450.00962284248.3510061
GTAACGG750.001480119438.68081
CTATTAT1501.129376E-633.84571
GTAATAT1352.1391055E-532.225043
CCGACTA1352.1391055E-532.225044
GGATAGA950.00471694630.5374741
AGCGTCT1204.0557174E-430.208178145
CTATATA1453.4787554E-530.010971
GTATTAT4350.030.0109671
GTTACAT2252.809611E-829.0025393
TAAGACT1555.4925214E-528.0669734
GTATAAC1306.4652157E-427.8948121
TGTAATA1907.1374507E-626.715342
GTCTATT1100.00965922926.3683872
TATACTG1409.988912E-425.8927255
TTAGACT1751.2539332E-424.859324
ACATGGT4101.8189894E-1224.755975
GTCCTAT6450.024.7377221
GTTGACA4450.024.4471361
GTGTTAC3001.6967533E-824.17551
Produced by FastQC (version 0.11.5)