FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1972-lungMR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1972-lungMR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586430
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG9230.15739303923741962No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC8420.14358064901181725No Hit
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG7540.12857459543338506No Hit
CCCTTGAAGTAGCCGTTCGGGATGGTCTCGATCCTGTTGCTGTCCAGGTA6950.11851371860239074No Hit
GTAGAGGGATGACCTCATGGTCCAGGTGACACACCTGCAGGGAGGAGACA6150.1048718517129069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGCT1900.049.6048556
TAGTCGG803.7329333E-545.3121268
TATAGAT700.001054554241.4423682
GTATATA1354.947142E-737.6115261
ATATATC1354.9570735E-737.6019023
TGTAATA1553.45608E-837.4318122
TACGAGG800.002036011536.2497027
TATACTG1604.584399E-836.2497025
GTAATAT1251.2620543E-534.8086173
CTATATA1251.2642684E-534.7997139
GTTATAC850.002737739134.1260953
ATACGAG850.002741175534.1173676
AGCGAAA3050.033.295451
TACTGTA1204.0562148E-430.2080867
GCGAAAG3550.028.6010682
TAGGCTC3301.8189894E-1228.5603717
AATATTA2054.0346276E-728.292455
ACGAACC2054.0346276E-728.292455
TCTGTAG3950.027.5384643
ATATTAG1606.8237816E-527.1872776
Produced by FastQC (version 0.11.5)