FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1972-lungLR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1972-lungLR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981546
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG13680.13937197034066665No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC11660.1187921910944571No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT11280.11492074747388305No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG10760.10962298251941324No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG9900.10086129432548246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1050.069.061491
TATACTG3750.040.5998845
TTACGTG1807.2759576E-1140.281772
CGACCCG1502.5995178E-838.666557145
AGCGAAA3700.037.2372131
GTAATAT3000.036.2572861
GCGAAAG4350.031.6698022
AGTCGAA1204.055638E-430.2113252
GTCGAAG1453.487969E-530.0029743
CGAAAGC4650.029.6265893
GGGTACT1000.00607448628.9999187
GTTGACA7900.028.4550861
AACCGAT1306.479107E-427.8873792
GCGTTAA1306.4829685E-427.8845399
ACATGGT8150.027.5766095
ATTATAC2405.266338E-827.1901933
CTACTGA12300.027.1137436
AAGTCGA1358.0731226E-426.8572481
CCGATGA1358.082739E-426.8517744
TAGCATG14450.026.594332
Produced by FastQC (version 0.11.5)