FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1972-lungLL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1972-lungLL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997050
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG11130.11162930645403943No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC10180.10210119853568025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC2501.8189894E-1234.8048023
AGCGAAA3750.029.0127321
GCGAAAG3800.028.6238062
CGAAAGC4500.027.392673
TATATAC2954.820322E-1027.037633
GTATTGA1650.002594233521.9793431
CTATTAC2003.0938332E-421.7595521
ATTGTAC1700.003090312721.3264733
TACATAG2857.0956085E-620.3516455
GTACTCT2556.9554415E-519.902716
TTCGACA1850.005044303419.5963174
TATTATA3351.3120352E-619.4812772
GTATTGG4102.1556843E-819.459761
TATAATA5605.456968E-1219.4232982
TATACTG3751.8074388E-719.3340635
GTGTTAC7600.019.0873241
GTCCTAG5356.548362E-1118.9802931
GCTATAT2701.07553715E-418.804551
GTAGTAT6950.018.7852231
ATATACC3501.9973832E-618.6444954
Produced by FastQC (version 0.11.5)