FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1971-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1971-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681358
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC8400.12328320794648334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCGT450.0096380648.3330426
ACGAACC1151.4109537E-744.1334045
TTAGGTA1903.6379788E-1241.979594
GCGAAAG2600.041.832812
AGCGAAA2700.040.2923161
CACGAAC1458.683437E-735.0049254
GTTACGT850.002736528634.1299631
TAGTACC1956.9758244E-933.4662484
GGATTAG3300.032.9664421
CGAAAGC3550.030.6381133
TATACTG2859.094947E-1230.528375
CCGACTA1204.0533594E-430.2125854
CGAACCC1703.0100018E-629.8527586
CTATGCG1555.4926175E-528.0684643
CCACGAA1855.806214E-627.4362933
GTACCTA1855.8128444E-627.4322666
CAAGACG2156.112259E-726.980544
GATTAGG3255.0931703E-1126.7729992
TAGGTAG4001.8189894E-1225.3767095
GTAATAT2401.5918249E-624.175391
Produced by FastQC (version 0.11.5)