FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1971-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1971-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899691
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC40620.4514883443315539No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA37040.4116969048262126No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG36250.4029161123096708No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT35710.3969140516021612No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT35630.39602485742327087No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG31080.3454519384988846No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC30180.33544850398636866No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG28010.31132911188396906No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA27000.3001030353754789No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC26550.2951013181192209No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG25950.2884323617775436No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA24180.2687589405695956No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT23730.26375722331333756No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA21870.2430834586541379No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC20890.23219082996273166No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG20580.22874520251953168No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT19380.21540728983617707No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA19190.2132954536613126No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA18150.20173592933573858No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC18010.20017983952268056No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT17860.19851260043726124No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT17400.19339973390864199No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG16310.18128446322126152No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG15860.17628274596500354No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG15150.16839114762735205No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC14920.16583471436304242No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA14810.16461207236706824No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA14360.15961035511081026No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC13980.1553866827610813No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT13860.15405289149274584No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC13390.14882887569176528No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT13360.14849542787468142No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC13310.14793968151287495No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT13300.14782853224051368No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG13250.14727278587870726No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC13050.14504980043148147No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG13040.1449386511591202No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA12700.14115957589883638No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG12370.13749164991091387No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC12240.1360467093702171No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG12130.13482406737424293No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC12130.13482406737424293No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG12090.1343794702847978No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA11780.13093384284159784No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC11240.12493178213408827No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT11200.1244871850446431No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG11070.12304224450394635No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT11060.12293109523158507No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA11050.12281994595922378No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT10900.12115270687380446No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG10640.11826282579241094No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA10550.11726248234115937No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG10440.11603984034518518No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT10440.11603984034518518No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA10170.1130388099914304No Hit
CTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGTTC10130.11259421290198524No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT9970.11081582454420462No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA9650.10725904782864339No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA9630.1070367492839208No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC9580.10648100292211438No Hit
ACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGGCTCCC9550.1061475551050305No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC9270.10303537547891443No Hit
GCACTACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGG9200.10225733057238541No Hit
ATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAGACACA9140.10159043493821768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGC100.007077971145.087481
CGCAATT550.0118.63535145
GACTTAA203.8660597E-4108.749084145
GCAGGTA5550.0103.231762
AGTAGAA8800.0100.5721
GGTACTG5750.097.091535
GCCTGCC353.4372322E-582.856445145
GCGAAAG2650.082.102162
CAGGTAC6800.081.0422443
GTACTGA7700.076.2655946
CGAAAGC2900.075.011873
TTTCGTT608.031202E-872.49939145
TAATCCA5700.069.959445
AGGTACT8250.068.548694
GCTTTAA5850.068.2035141
ATATCAC1550.065.494243
ATCACTA1550.065.486965
GAGTTTA350.003566505962.142334145
AGAAACA14350.061.1352844
AACACTG8050.060.3444565
Produced by FastQC (version 0.11.5)