FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1970_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1970_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAACAGACATCTGACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGCT40.18206645425580337No Hit
GCCGAAGGCAGAGGCTTTAACCCACTGAGCCACCCAGGCGCCCCTCTGTG30.13654984069185253No Hit
CAGTTGTAGTGCCCTTCATTGGCTCTTGTTACAGAGTTTGGATTTTTTCT30.13654984069185253No Hit
TCTAGGAACAGGATGATTTTCTCACGCTGTAAGTGGGGAGACCAAGCACT30.13654984069185253No Hit
CTAGTGTTGAGTCCATTTCACAACTGAACAACTCTTGCACTGAAAAATTA30.13654984069185253No Hit
GTAGAGGGATGACCTCATGGTCCAGGTGACACACCTGCAGGGAGGAGACA30.13654984069185253No Hit
CATCATAAGGTATGCAAAAAAAAAAAAATTGGAAAGTATAAAGGGAAAAC30.13654984069185253No Hit
ATCACATCATGGGTCTGTGGGCACATCATCCCAGCATGATTGTGTTAGAC30.13654984069185253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGGT100.0066095586145.04
Produced by FastQC (version 0.11.5)