FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1970-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1970-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444161
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC6520.1467936176296433No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG4490.10108946980937092No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG750.001482525838.666524
GTATTAT3550.036.76871
GCGAAAG2252.0008883E-1135.452291
TATTATC2008.907591E-932.632222
TAATACA3600.032.2220954
CTAATAC1153.1572764E-431.5251663
AGCGAAA1851.636945E-731.358291
TATAATA2558.731149E-1131.2814312
GTAATAT1000.006064309729.0064181
GTTAATA1000.006064309729.0064182
TTAGAGT1804.692385E-628.1943344
GAGCAAC3900.027.8845086
TCTAATA1855.7966136E-627.4385032
TATAGTA1100.00965548526.3694732
ATCTAAT1658.395584E-526.369471
ATAATAC3057.075869E-1026.1503833
CGAAAGC3108.5310603E-1025.73152
TAGTACC1701.03005616E-425.5881354
GTCCTAT4900.025.1586281
TATACTG2651.3727913E-724.6225455
Produced by FastQC (version 0.11.5)