FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1970-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1970-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604423
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA12880.21309579549421515No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10520.17405029259310117No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA8240.13632836606151652No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC8170.1351702367381784No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT6960.11515114414904794No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC6790.1123385443637982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTT350.003566264962.140907145
AAGGTCG2057.2759576E-1238.9012229
GTAGAAA5150.036.618972
TCGCCAT1001.391055E-436.24886145
TTTAAAC6850.033.878763
AAACAAT7050.032.906776
ACAATTC6750.032.221218
GTATATA2051.1265001E-831.85991
TTTCGTT1453.488881E-529.999058145
TAAAAGA6850.029.6390114
ATAAAAG7000.029.008693
TTAGGTA2252.809611E-829.003894
TACCATG2502.4429028E-928.999097
GAACTAA7400.027.4315729
GTACTGC1606.7791945E-527.2136631
GCTACTA1606.825198E-527.1866479
GTGATCG1358.0804725E-426.851019
GTAATAT1907.0960723E-626.736231
ATTAGGT2456.410846E-826.6406353
TACGGAG1100.00967009426.362815
Produced by FastQC (version 0.11.5)