FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1970-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1970-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569776
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA75581.3264861980848615No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG64191.1265830782623347No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG62741.1011344809188173No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT58821.032335514307377No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA53390.9370349049451012No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG52890.9282595265507849No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT41260.7241442250989862No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC41160.722389149420123No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT40220.7058914380388083No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT36050.6327047822302098No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA32960.5784729437533346No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC31150.5467060739659093No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT28110.49335177332846597No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG27820.48826205385976246No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA27120.47597652410771957No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT24150.4238507764454803No Hit
TCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATA23890.41928757968043584No Hit
GTATAAGGGAATGTGGTACTTATTGCATTTTGCGCTGGCACTTTTAAGAA22480.39454101260846364No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG20940.3675128471539693No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG20690.3631251579568111No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC20600.3615455898458342No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA20340.35698239308078966No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAA18270.3206523265283199No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG17910.3143340540844121No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG17830.3129299935413215No Hit
ATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTT16840.2955547443205751No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG16630.2918690853949622No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT16310.28625284322259975No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT16180.2839712448400775No Hit
TCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCA15500.27203673022380725No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG15100.26501642750835414No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG13810.24237595125101793No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT13780.24184942854735894No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA13750.24132290584369998No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA13420.23553115610345118No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC13300.23342506528881524No Hit
GTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAA13210.2318454971778383No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT12970.22763331554856645No Hit
GGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGA12960.2274578079806801No Hit
CTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGA12830.22517620959815787No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC12810.22482519446238525No Hit
CGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAA12630.2216660582404313No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA12480.2190334447221364No Hit
CACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAA12480.2190334447221364No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACT11940.20955603605627474No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC11750.20622139226643454No Hit
TATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGA11520.202184718205049No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT11420.20042964252618575No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG11390.19990311982252673No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT11380.19972761225464042No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT11370.19955210468675408No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA11370.19955210468675408No Hit
GAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTGACAAA11360.1993765971188678No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA11360.1993765971188678No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC11200.19656847603268654No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC11070.19428687765016425No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG10580.18568700682373424No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA10330.18129931762657606No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG10250.17989525708348544No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT10040.17620959815787257No Hit
GGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCG9850.17287495436803235No Hit
TTACCAACACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCT9850.17287495436803235No Hit
ACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTTAA9790.1718219089607144No Hit
GAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAAT9570.16796074246721518No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA9490.16655668192412457No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA9490.16655668192412457No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA9190.16129145488753474No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG9190.16129145488753474No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTC9180.16111594731964843No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC9120.16006290191233047No Hit
ATCTCGCACTCGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCA9110.15988739434444413No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG9080.15936087164078516No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA8870.15567521271517226No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA8860.15549970514728595No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC8600.15093650838224143No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA8600.15093650838224143No Hit
ATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATA8540.1498834629749235No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA8400.1474263570245149No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTG8390.14725084945662856No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA8390.14725084945662856No Hit
ATCTTACGAAAGGGGAGAAAGCTAATGTATTGATAGGGCAAGGAGACGTA8220.14426722080256102No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT8220.14426722080256102No Hit
CTGTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAG8110.1423366375558114No Hit
ATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCA8060.14145909971637977No Hit
CATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTTAAT8010.14058156187694815No Hit
TAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTG7960.1397040240375165No Hit
GTTCATTGATGCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGG7950.1395285164696302No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG7950.1395285164696302No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA7770.13636938024767628No Hit
ACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATT7510.1318061834826318No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT7500.13163067591474545No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA7460.13092864564320014No Hit
CTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTC7420.13022661537165484No Hit
TTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAA7320.12847153969279154No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA7260.12741849428547358No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT7200.12636544887815565No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT7190.1261899413102693No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA7170.12583892617449663No Hit
TGTATTGATAGGGCAAGGAGACGTAGTGTTGGTAATGAAACGGAAACGGG7100.12461037319929234No Hit
TCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATC7050.12373283535986072No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAT7050.12373283535986072No Hit
GTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT7030.12338182022408806No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG6980.12250428238465642No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG6930.12162674454522478No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA6850.12022268400213418No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG6760.11864311589115722No Hit
ATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAAT6720.1179410856196119No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC6660.11688804021229394No Hit
CAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCT6550.11495745696554435No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT6510.11425542669399903No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT6500.11407991912611272No Hit
AACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAG6500.11407991912611272No Hit
CCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAG6420.11267585858302209No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG6370.11179832074359047No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC6320.11092078290415883No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC6290.11039426020049985No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG6210.10899019965740922No Hit
GCTGTTCTGTTGACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCT6150.10793715425009126No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA6080.10670860127488697No Hit
GTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGACAAAAGATATGGACC6000.10530454073179635No Hit
TCGCAATCTCGCACTCGCGAGATACTGACAAAAACCACTGTGGATCATAT5960.10460251046025104No Hit
GAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAA5840.10249641964561512No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGT5820.10214540450984246No Hit
GAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATAGGGCAAGGAG5710.10021482126309288No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTA100.0070901434144.99948145
GCAGGTA5450.0118.404462
CAGGTAC5500.0117.328063
AGCAGGT5950.0109.6923451
AGCGAAA700.0103.598331
AGTAGAA11250.0103.1378861
GGTACTG6400.0100.8199545
GTACTGA7300.089.383246
TACACTA259.384797E-486.999685
ACTGATC7900.084.430088
CTGATCC7800.082.724069
GCGAAAG900.080.562332
GGTCAAA2200.075.815141
TACTGAT8850.073.7285467
TTACTAA300.001933320572.518831
TAGATCG300.001935341372.49974145
CTTACGG300.001935341372.49974145
GCTTTAA9150.072.122551
ACAAGGC7750.071.096528
ATAAAAG18700.068.2410353
Produced by FastQC (version 0.11.5)