FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1969_lungMR_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1969_lungMR_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATATGACCTAAGTTGGAGAACGTTCCAGGGGCACTTGAGAGGAGTATA21.2048192771084338No Hit
GGATCACCACTCCCAGGTCTCCTACTCAGCTGCTGGGTAGAGTCGACAGC10.6024096385542169No Hit
GTTCTGGAACGTGCCATTAGATCAGGTCACGGCCGTGTTTGCCGGGGCTG10.6024096385542169No Hit
GAACAATCTGGCAGCATTTAGGAAAAACAGGTGTGCTCACTCCCTTTCAC10.6024096385542169No Hit
CTCCTCAAATCAGAAGTGATTAAAACACAGCCACACTGACTCTGAATTAT10.6024096385542169No Hit
ACTCTGTACTGTAGCCTTGGTGGCCTTCTTTGATCTCTTTACCAGGCCAC10.6024096385542169No Hit
GATTAGAGCTTAGCAACAAGGTTTCTAACCTGGAGTACGCGGAGCCATAG10.6024096385542169No Hit
AACCACAACTACTGGCAGGCACACCCTTGTTAAGAGTTCTTCAAAACTCC10.6024096385542169No Hit
GTGAGGACTGTGGATTTACTGCAACCCAGCAAGGCAGCTGGGTTCCCCCA10.6024096385542169No Hit
GGTGTGACAGTAGAAATAGAAATTGAGCGAGCCCCGGAGCGGTCCTGGAC10.6024096385542169No Hit
CTCCAGAACAGAAGGGAAAGGGTTGCGGTGACCTAGTGACGGCGGCTCAG10.6024096385542169No Hit
GTCCCACATACCCGATCAGGCAGTGTGACCAGATCCTCTTCCCTCAGCAG10.6024096385542169No Hit
ATTAATTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTTTG10.6024096385542169No Hit
TACTACTACTTTATCCATTCATCCGTTGGCAAAGGTTCCATACCAAGATC10.6024096385542169No Hit
CCTCATTTTAGATAGTTCTGGAAATCTTTGCAAGGGCTCTAAGCTCACAT10.6024096385542169No Hit
GATCACTGCACACCGCCCATGCCCTGGGATGGGGAGTAATGGGAAAATGT10.6024096385542169No Hit
TATACCAAGCTTGAAAAACTCAAATTTTAATTTTTGTCACGCTGAAGCCC10.6024096385542169No Hit
ACCCAGGTGCCCTCTTGTGACCATTTTTAAATCGCCTTCAATAAATATGA10.6024096385542169No Hit
TCTCAGTACAGGCGGGTAGAGAAACAGGGAAACAAGAGGCGGTCTAGCAC10.6024096385542169No Hit
CCTTGGGGGGCATCTTCCTCAGGATATTGTGGGCCAGGTTGGGCTGCATG10.6024096385542169No Hit
CAGCAGAGAGCCCCATGCTGGACTCCATCCCAGCACCCAGGATCCAGACA10.6024096385542169No Hit
CCTAACATCCCTATTCTAGGAACAGGATGATTTTCTCACGCTGGAAGTGG10.6024096385542169No Hit
CCTTCTGCCTGGATGGCTCACCCTCGCCAGAGCAGGGTAGGTGTGTGGCT10.6024096385542169No Hit
CCAGTAGAGAGATGAAATAAGAAATCAAAAACTTCCAACAAGCAAAAGTC10.6024096385542169No Hit
CCCCACTGCACACCTGCTCCGTGGTTGTGGGCAAGCACCGTGGCGCCTTG10.6024096385542169No Hit
GGGCAGCATTGTGTGAGGGCATCGGTTCACGCTGGACGTTCTCCTGCTCT10.6024096385542169No Hit
CATTTTGTGCCGAGAAAACCAGCAAATTCAACAGACAGTGCCATTCTTAC10.6024096385542169No Hit
CAGAGGAAGGCTGTGTCTGACAGCCGGGGTGCCCTCATCCCGGCAGTGCA10.6024096385542169No Hit
CCCCCCACGCATCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGC10.6024096385542169No Hit
GAGACGATACTCTATGAAGGACACAACAACAACACCTCAGATCTCACTGC10.6024096385542169No Hit
CCACACGTCACCGTGACTACAGTGAGGATAATTATATCTTGCTTCTCAGA10.6024096385542169No Hit
CCCCAGTACCACAGAATCTAACTGTATTTGGAGATATGGTTTCAAGGAGG10.6024096385542169No Hit
CCCCCGGGACTGCTACTGCCCCCCGGATTTCCCGTCCGCCCTCGACGGTG10.6024096385542169No Hit
TTCCTCAGCAAGGCTTTTGGCGTTCGCTCTGTCGCCTCAGGGAAGAAGAG10.6024096385542169No Hit
CCCTGTGGGATTGTGGGATTAGAGATTCCACCCAGATTTCAGAAGGGCGG10.6024096385542169No Hit
ATGGACGGATGGACAGACGCTTTTCCCCGGCCCTCCAGAGGCGCCCTACT10.6024096385542169No Hit
GTGCAAATTAGAAGAATAGATGGAGTTTCTTGTACAGTTGATGAGTCAGC10.6024096385542169No Hit
AAACTACAGATGGTGTGGCACTAAATAGCCTTTAAAGGGACACTAGTTTG10.6024096385542169No Hit
GGCTGGCGCGTGGGGGGGGGCGCGGGGGGGTGCCGCGGCGGCGGGCGGGG10.6024096385542169No Hit
ATGCCTCCTCCGTGTCTGCCCCAGGGCTGGGGCTGGCGCCTACAGATGGC10.6024096385542169No Hit
AGACCTCCCAAGTATCTGTGATGAACATAAACATAAAAATCCTCACCCAA10.6024096385542169No Hit
TTGTAATGTGAATGGCTACGAAACAGGCAAAAATTAGAAATGCAGGTAAA10.6024096385542169No Hit
AAACAGTTGAGATAAAGCCTTTAAAAGCTCCATCAATAACCACATCTCAA10.6024096385542169No Hit
CTGTAAGTGGGGAGGCCAAGCACTTGAGAGGCTAAGGGTCTTCTCCAGCA10.6024096385542169No Hit
TAGTCCCAATATCTTGCTCCAGAACCCCAAGCTTCAAGACGCTGAGGAAC10.6024096385542169No Hit
CAGCATTAGTTTCATATGTAGTGGTAATCAGGTGTTCAGTTGTGAATAAC10.6024096385542169No Hit
GGTGTTCACTAATTCAACTCAATACTGACAATAACTATCCAGAGTTAGTA10.6024096385542169No Hit
TCAATGATCTGCGTCCTGTCTTAGGTTGGTTTATTTCCCCCACCAATCTT10.6024096385542169No Hit
GAGCAAAACACCTGACTGGGGCAGCTAATCTCCAGTGACAGAGGCTGCCT10.6024096385542169No Hit
TAGTAACACTGACCTCACTGAGAAGGATTCGGTTGATGATGATTATTAAG10.6024096385542169No Hit
CTTTATTATGTATTGCGCCTTACCAAATCTAAGCTGTCCAGTCACAGCCC10.6024096385542169No Hit
CTCCCTCTTCACTAGGAGCCCGTGAGGACAGTGCTGGTGATGTCTCCTGA10.6024096385542169No Hit
GGGTCCTGGGATCGCGCCCTGCATCGGGCTCTCTGCTTGGTGGGCAACCT10.6024096385542169No Hit
GGCAATGTACATGAACCCGAAAATGGTCAGCTGTCATTATCAGGGCTGTA10.6024096385542169No Hit
ATTAAAAGTACGTATGTTAACATGTGAGCCTGACAATGTGTCATGGGTGC10.6024096385542169No Hit
CTTAACGGACTGAGCCCCCCCGGCTTCCCCATAAACCACCTATCCTGACG10.6024096385542169No Hit
GTTCCTAGCACTGTGGGTATGCTGGCTGCACGCCCAGCGATGTGTGATTC10.6024096385542169No Hit
CCTATACTACGTGAGCCCGGAAGTTGCCCCAAATAAAAATGCACATCCTT10.6024096385542169No Hit
ATCCTAACGGGTCCTTGCAGACACGGAAACACGCATGTGTACATGGTTTT10.6024096385542169No Hit
GTTATATGAGAACAACATTATTCCCTAAGTGGGGGTCCCAGTCAACACCC10.6024096385542169No Hit
ACCCATATGCATTTGTGAATATTAAATGAACTTATTTATCTGAGCTGAGC10.6024096385542169No Hit
CTCCTGACACGGGCGGATCTGGAAAGTCGTGTGGCCCAGGAAAGGGAATG10.6024096385542169No Hit
ACCCAGCGTAGAGCACGCCCACAGGCAGCTGGCACCCGCCCCTCGGGGCC10.6024096385542169No Hit
AAATAGCGCATATATGAACCAACCTAAGAGCAGCAATCAGTACCAATACA10.6024096385542169No Hit
GCTTTTCCCAAGTCCCAACCCCACATGGCTATCAGAAATCTAGCACCCTT10.6024096385542169No Hit
AGGTGAGAGTCTTAACCATTGCTGGAATAGGACAATTGAATTCTCGCTAA10.6024096385542169No Hit
GGTCTGCAGGGGCCACAGAGGCTATGGCGGCCCAGCAGGCCCCTGCCTGG10.6024096385542169No Hit
CTCCGTCCTCTGTCTTGCAAATGCAGATTAGAGCAGCATTGCTGCCTTTC10.6024096385542169No Hit
GAACAAGTGCAACCAAGATGGGAAGGGAGATCCAGACTCAGACCCTGGAA10.6024096385542169No Hit
CAGTTTGCAGAGAGGCCTGAACAGCTCTCCCTTGGTCCCAGAATCAAAGT10.6024096385542169No Hit
AAGGTGGGGAATAGGATCAGCAAGAAGAAGGGCAGACGAAATGAAGTCAA10.6024096385542169No Hit
ATTCTAGTTCTCCCACAAATACATCCTATAAAATGCCAGTCTGAGAAGAT10.6024096385542169No Hit
TGCCCAAGGTCACACCAAGAACAATTAGCAAAGCAGAATTTCAACAGATC10.6024096385542169No Hit
CTCTATTACTTTGCTTAAAGTGGTTTATCTTTCACGTTTCTCATGTGCTC10.6024096385542169No Hit
GAAATACATTTGTACCCTTTGGTAAATGTAGCCACTGCCTCAGCCCCATG10.6024096385542169No Hit
CCCTGAAAGTATAATCTCAAGGTGAGAGCCCCAGCGGGGACGGTGATATA10.6024096385542169No Hit
GCTGTGAATCAAGCCGAAGGAAGCACATTTCTTATATGGGGAAAGCAGTA10.6024096385542169No Hit
ACCACACTTTGCTTATGCACTTGCCATTGATGGATATTTGCAGTTTCTAC10.6024096385542169No Hit
AAATAAGGGGGAGATTAAGGCAGATTTATATGTAGAATCATGATCTACAA10.6024096385542169No Hit
GTTCAAGGTTTCTCCTTCCATGAAATGTCCCAATTTTTAAGTATCGTGAT10.6024096385542169No Hit
TCTCTACACCCCCCATGGGGCCTGAACTCACCACCACAACATTGGGTCGG10.6024096385542169No Hit
AGCCGCAACCTCCGGAAGGTCCCTGTCATCCCCCCACGCATCCATTACCT10.6024096385542169No Hit
TAGTTGATCTGGTGGCAGCTCACCTCCATCTATAAACACATGCACACGAC10.6024096385542169No Hit
GGTTTACGATGTGGCCCTGAGGCCAGGTCTGGAGTGGCTGATGGCCTAGG10.6024096385542169No Hit
CGTGTGATGTGTGCGGTTCCCTGCGGCAAGGCCCCGCATGGCTCTGTCCA10.6024096385542169No Hit
GTGATGAGAGACACAATTGGAAAATATCACAAGGGCAGTCAGAAGGTCAA10.6024096385542169No Hit
TATATGAACAAACCTAAGAGAAGCAATCAGCACCAATACCATAGGGAACT10.6024096385542169No Hit
AAACACACACCAAGAATTATTCTGGAATAAACCCACTATAACACACACAA10.6024096385542169No Hit
AAGCAGATGCAGGGGACTCAGGCCCTTAAGCACCCTAGTATCTATCTGGA10.6024096385542169No Hit
AATCAGGGTTGGGTCCTTCTTCCATCACCTAGTGTTGACTGGGATTGATG10.6024096385542169No Hit
GGGCTTGATCTCAGGGTCATAAAAAAAAATGATGGAAAATTTAGTTTCAG10.6024096385542169No Hit
CTACCTGGATTCTGGATTCTTCTATCCTGGCAAGCTTGGGAGGCAGCATT10.6024096385542169No Hit
ATTGTATAATGAGTAGAACTCTGACAGGTAAGAAGGGAAGAAGGATGTCC10.6024096385542169No Hit
GAAGCATCCATCAGCCAATTTTCTTTTCGTTGAAAAATCAGTAAGTCACA10.6024096385542169No Hit
GTAGGAGACAGAGTGTGGAGGCTGGCTTCTGCCCGGGGCCCCACCCCCGT10.6024096385542169No Hit
GGGTAGGCCCTAACACCACGCTAGATGGAAGGAAGTACTTTGCTCTAAGG10.6024096385542169No Hit
TCTTTGAAAAGAACAAAGCTGACAGAGTCTCAGCCAGATTCAAAGAAAGA10.6024096385542169No Hit
TGCTTTTACCTTGTCCAAAATGTCCTACTAATGGAGTCACCAAGCACGTG10.6024096385542169No Hit
AAATAAAAGTTCCTTTATCATGAAACATTATTTCCTCTTATGGAATGACC10.6024096385542169No Hit
AGGCAAGGTTGGGGAAACCCTTGAAGTAGCCGTTCGGGATGGTCTCGATC10.6024096385542169No Hit
CATATGTGCAAAGCAGTAGTTTTAACCTACCCACAAAAATCTGAACAACC10.6024096385542169No Hit
GTTTAGTACACATCCAGTTAACCAAACCACTGTTCAGCATCTAATCCTGC10.6024096385542169No Hit
ATTTGTAAGTTTCTCCCTAAATCACAAAAGTTCCATACCTAATTATAATA10.6024096385542169No Hit
GTCTGGAGGTCCATCTGGTGTCAGGGATGCGGATGTGGCGCCTTCCAGCT10.6024096385542169No Hit
GGTTAATTCCGAAACCACGCTACACGGACACTGCAAATAAACAATGAAGT10.6024096385542169No Hit
CCGTCAGGCCAAATGACCAATCATGTATGAGGATGAAGATGACTTGAGAT10.6024096385542169No Hit
GCGCCAGGGCAAACGGGCACAAAGCAAGGTACAATAGGTACTGAGCTCTT10.6024096385542169No Hit
ATTAATAGCACATTTATCATGACAGATGGTAAATTCACTTGAGTTGTGGA10.6024096385542169No Hit
GCATCAGCCGCACTGGTGGGGGCCTGGAGAGCGTGACCTCCCAGCACCCC10.6024096385542169No Hit
TCGCAGGACTTCCCCCATATGGCAGTAATAAATGTCTTCAATGTTAAACT10.6024096385542169No Hit
GCATAGTTGTGCCTGGGGTGCTAAGGACTGGATGCCTTCCTGCATAGCTT10.6024096385542169No Hit
GCCTGCGGTGGCTCCCCCTGGCTCACAACCGGATTCGCACGCTCGACCAG10.6024096385542169No Hit
TTGTATGTTCGAGAAGCAATGTTCTATAAGAGGAGTTTTTAAAATGTCGT10.6024096385542169No Hit
GGGAAAGATCCATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATTCAGT10.6024096385542169No Hit
GGATATGGTCTTTGGAGATGAATTTTTTGTGTTTATTTACTGAGGTAATA10.6024096385542169No Hit
CTCTATGTGCTCTCTACCTCTGTCATTACATGCATATTTGCATGACAACA10.6024096385542169No Hit
CTTGCGGCCCTGGAAGGTGTCGGGCTGGAAGACACCATCGCTTAGCGTGG10.6024096385542169No Hit
GAGCCAGTGGCAGCCAATACTGAGCCTCCGGACGTAGAGGAGTCTTAAAG10.6024096385542169No Hit
ACTGGAATCGGAGTCCCGCAAACCATCCACACCTCCCAGCCACCTTGGCC10.6024096385542169No Hit
CTTCAGCTTTTTCTTTGCCTTTGTTTTTTTCTCCTTTCTCCCTTTTTTCC10.6024096385542169No Hit
GACCCAAGACGGGCGCGTGTCTACAGCATTTTCCGTTCTTTCCAGGACCT10.6024096385542169No Hit
CATACACTTTCCAAGAGAATTGTATACTAGAGAATGGGATACATCTATAC10.6024096385542169No Hit
ATGATGCAACATTTATAAATATTTACCCACCCAGCATGGAGCAACTAAAT10.6024096385542169No Hit
CTCTTCCCTGGAGGGCCACGCTTCCTGCCCGTGCCTCTTCCTACGCCACC10.6024096385542169No Hit
AGCCTGCTCTGGTGAGGGTGAGCCATCCTGGCAGAAGGCACACATCAGCT10.6024096385542169No Hit
GGCCTGAGGTGGATCAACCTGGATAACAACCGGATTCGCAAGATAGACCA10.6024096385542169No Hit
AATCTTACTTTCAACAATGACTTGAGCAACTAGACATATTAGCAAAGAAA10.6024096385542169No Hit
CCTTGGGGGGCATCTTCCTCAGGATATTGTGGGCCAGGTTGAGCTGCATG10.6024096385542169No Hit
GGGCCGAGGGCACCTCTTCGAGCTGGTTCTTCTCCATGTAGAGGAATATC10.6024096385542169No Hit
AGATTAAATAAGAAATCAAACACTTCCAACAAGCAAAAGTCCCGGACACC10.6024096385542169No Hit
GCAGGCAGATGCAGGGCGAATCCTTGTTAGCTGGCCCAGGGTCACGTCCT10.6024096385542169No Hit
ACACAGTCCTCCAGTGGATGAGAGCCCACCATTATATTCTCAATTTTTTT10.6024096385542169No Hit
CATATATTCTGAAGACGAGGTGAGAGTCTTAACCATTGCTGGAAGAGGAC10.6024096385542169No Hit
CTCATGGCCGTTCCGTTTCCCTCCAAATCCAATGCCTGCATGTTAACAAT10.6024096385542169No Hit
CAAGATGTACCACAGGAGTGTTGTCCTCGTATTCGCTGTTAAGTGGCTGC10.6024096385542169No Hit
GGAAGGAGGACAGGCCCGTGACCTAGGCAGGCTCCTCCTGCACCATCCGT10.6024096385542169No Hit
ATTCCTTAGCCTCACCTGATAAAGTCCATACTAACTAGTCAGAAGGGCTA10.6024096385542169No Hit
GAGAAGGGCCCTGTGGGTGTTTATGGCAGCTTCATTCCCATTGCTCCCCG10.6024096385542169No Hit
GAGCTTCCCTCTGTGCTGGAGGAAGGACTGCTGGCAGGTTTGTTTACTCC10.6024096385542169No Hit
ACTTACTCAAGTGTATTTATAACCTCATCACAAATGCTCATACTACTCTT10.6024096385542169No Hit
GGAATGCTACTGCCCCCCGGATTTCCCGTCCGCCCTCTACTGTGACAGCC10.6024096385542169No Hit
CCGGTGGACAGGGGACTAGGCCAGTGCCTTCCCCAGCCATGAGGAGACGC10.6024096385542169No Hit
TCCCAGGGCTGCAGAATGCCATGGGACTGCCAAGGGGAAGATTAACAACC10.6024096385542169No Hit
GAGATCAGAGTGAGGACTGTGGATTTACTGCAACCCAGCAAGGCAGCTGG10.6024096385542169No Hit
GAAATACTTTGAGGTAAATGAAAAATTAATACAATGAAACAACATTCACA10.6024096385542169No Hit
GTGAGAAGATCCTCTCTCTGGGGTGAAGGAAGGCTTCCTGGCCAAAGCTG10.6024096385542169No Hit
GGGTAAATCTGAAGCTAGGATTTAGAGCAGAAAATGCTGACAATCAGCCC10.6024096385542169No Hit
CGGTACGCCCTCCTAGGCCCCAAGCCGAGGCCATCAGCCACTCACGACAT10.6024096385542169No Hit
TCCCAGGGCCGCCCACTCGGTCTCCCTCCCTCGGGGGACTCACCAGCACT10.6024096385542169No Hit
CTGTAGCATAAATCCTGAAGGAATGAAATCCAGTGTACCGGTGGTGTAGC10.6024096385542169No Hit
CCTTCGTTTTGTGAATGCGGCACATTAGTTTGATTTGTGGATGTTGAACC10.6024096385542169No Hit
TAAGAATGGCTTTGCAGAGCCTGATAAGGACACAGCACTGGAATGGGCAC10.6024096385542169No Hit
ATTCAGCTTTCTGATGATCAATCCGTTAGCAATTCAAGTGCTCCGGACGA10.6024096385542169No Hit
GGGTCTAGGCTGTGGCGGGAGCTCACTGCTGTCCTGCACGGTCCCTGCAA10.6024096385542169No Hit
ACCTCCTTGTGTGCAGCTAAGGCTATGTGTTCATTTCCTAGGAGCACTAT10.6024096385542169No Hit
CGAGACGTCTCTTCCTTTTGCTGCTTTTGGATGGAGCGTTCTGTGTGTAT10.6024096385542169No Hit
AATCTATTGATCATGGCATTCCCTCCGTGAAGCAAGCATGACCACGCCTG10.6024096385542169No Hit
GGGGCTGCCTGAGGGCCCTGGGCTGGGAGTCTAGAGATTCAGGTCTCAGC10.6024096385542169No Hit
CTTGCTACAGTGTTTGGATTTTTTTTCTTGTTCTACTCCATTGGAACTGA10.6024096385542169No Hit
GAACTGGACTCCCTGCATCTGTTCTTTATTTTGGTAGTAAAATACACATA10.6024096385542169No Hit
GGTGAAACACATGCAGGGAGGAGACACAGGAAGTTACTAGCCTGCTGGAC10.6024096385542169No Hit
GCCACCTGAGGCAACTCAGCCACCCATCAAATAAGATGGTGCTAACCCAG10.6024096385542169No Hit
CCCCACACCACCTCCTGGAAGCTTTCCCGACCCCCAGACGGAGACATGCC10.6024096385542169No Hit
GGCCGAGTGGGCCCAGACATTGAGCACCTGTTGTACCTAGTCCAAGCTGA10.6024096385542169No Hit
GTATTACCCCCGGCCGCTGTGACGGCCAGGGCCGGCTCTACAGCGTCGCA10.6024096385542169No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers