FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1969_lungLR_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1969_lungLR_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATAGGCTAGAATTTGTATAGGATAGCGGTGATTAGGTATGTGGGGC17.6923076923076925No Hit
GGCTGGTAGAACACCGTGTTGCCAGGAAGGTCGCTAGAAGGAAGACCCTT17.6923076923076925No Hit
CTTGAGAAGTATAGCAAGGTGTTTTGTATAAACCACATCTTCATGGTCAC17.6923076923076925No Hit
GGACACAGGGACGATGGTGTGCAGGTGGTGCTGGCCACGGCCACGGCCAC17.6923076923076925No Hit
GGGGTCCTCTGGCCCCACCCAGGCTTCCGGTCTCCCGGCACCCCTCCCCA17.6923076923076925No Hit
AAGTGCCCCTGCCGGGCGGGGTGGCACAGGTAGCGGTGCCGGCCCGCGCT17.6923076923076925No Hit
CAGCACGTCAGACACTAGTCACAGGGCCAAGTTTGTAACCGGTAAACTTC17.6923076923076925No Hit
CCTTAAAGGGCTTTGATGGGCGGAACACCGTACGCTGTCTGAAGACCCAT17.6923076923076925No Hit
GATGTGGATAAAAATGAAACTTGCTTTCTGCCATGGCAATCATCCCCTCA17.6923076923076925No Hit
CGCTGTAAGTGGAGAGACCAAGCACTTGAGAGGCTAAGGGTCTTCTCCAG17.6923076923076925No Hit
GGATCACAGAGCTCCACTGACTAATCGACTAGACTAATCGACTAATCGGG17.6923076923076925No Hit
AGAGTGAAGTGCTGCAAGTACAGAATGAATGAATGAAGGCATGAATGAGT17.6923076923076925No Hit
CTCTTGAACATCTTAAAAGCTCTGGCTTTTCCCCTTCTGAGGCTACTTAT17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)