FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1969_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1969_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467561
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC6130.1311058877879036No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG5620.12019822012528847No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGC100.0070898198144.998937
TATTCGA203.8615812E-4108.772462
CTATTCG350.003556365162.1822971
AGCGAAA1157.1035574E-637.8500941
GCGAAAG1301.6505699E-533.468452
TAGTGTA1255.149918E-428.9997869
GTACATA1358.046361E-426.8688931
GTATTAT5200.026.5071981
CGACTAG1958.74197E-626.0254486
TACATAG2001.06218395E-525.3802432
TAGCATG7150.025.3548872
TAGTGCA1500.0014931524.1664899
ACATGGT4550.023.9009235
CGAAAGC2151.8615474E-523.6070023
GCATGTT7800.023.2394924
CATGTTG6600.023.068015
ATATATC1902.1892661E-422.8970173
GTAGCAT8250.022.8629861
TTAGAGT1600.002172172522.6585044
TAGTGAT2303.1375894E-522.0650547
Produced by FastQC (version 0.11.5)