FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1969-lungUR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1969-lungUR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541196
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG6110.1128980997642259No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC5510.10181154332256706No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG657.320406E-444.631681
GTATAAC951.0260567E-438.171831
GGCTATG1504.3840875E-529.0105951
AGCGAAA2252.8021532E-829.0105931
GTATATA1255.1397533E-429.0105931
CTATATA1306.4652535E-427.89481
GTATAAA3403.6379788E-1227.7307131
TATAGAT1050.00769859827.624032
GTATTAT3900.026.0351471
TAGAAGT1409.988911E-425.8927424
GCATAAT1500.001490215324.1754951
TATATAC1851.8280659E-423.515583
GCGAAAG2802.3344182E-723.3077742
GTACCTA2502.268791E-623.2084751
ACCTATG2502.274266E-623.2020423
ATATTAG1902.1859493E-422.90311
CCATACT2552.7033766E-622.7449974
TATTATT5300.021.8907412
GAGAGTA2653.7747577E-621.8866969
CGAAAGC3004.543108E-721.7519133
Produced by FastQC (version 0.11.5)