FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1969-lungUL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1969-lungUL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823539
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAGACCTTGTAGTTTCTACTGATAGCTTCTATTCTCAGTGAAATGG16740.20326906193877886No Hit
ATTTTAGAGTGATCGCTTACACTGAAAAATATTTTGGCCTAAGTTTCATA10470.12713423407998894No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT10110.12276285640388615No Hit
GCATATACAAAACTGAATTCTTGTTCTTCCCCCAAATATCTGCAGAATTC8980.10904158758723023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG3050.049.917749
TAGCCCG450.00963644848.3359834
CATACCG604.9432326E-448.3359834
ATACCGC604.944713E-448.333055
TTAGAGT3250.046.8487244
TAGAGTG4000.039.8747635
AGTGATC4150.036.686538
CGAAAGC4500.033.83933
GTATAGG1102.4306559E-432.9663621
TATACTG3400.031.9851065
GCTATAT2252.805973E-829.0103991
GAGTGAT5250.028.9998287
GTAATAT5300.027.3683011
CTAAGAC13100.027.1231043
ACTCCGC2156.1227E-726.9765858
AGTATAC2705.5497367E-926.8565863
TAAGACC13500.026.8533254
TAACCTA2456.435948E-826.6324965
TGCAGCG3306.184564E-1126.3634825
AGTACCC1100.00967088826.3634829
Produced by FastQC (version 0.11.5)