FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1969-lungMR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1969-lungMR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657135
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG450.00963781848.333153145
GTGATCG1402.728484E-1046.606979
ATATACG951.9199215E-645.7893034
TACGGGG1002.7327733E-643.499847
CTAATAC1903.6379788E-1241.973533
TATACGG1053.822208E-641.428425
TAATACT2109.094947E-1237.976054
CCTATAT1601.8703104E-631.7307051
GAACCGA1153.160996E-431.5216246
TACACCG1453.488679E-529.999895
TATATAC3650.027.8081193
GTATTAT5650.025.6733911
CTATATA2301.1007251E-625.2211382
TAACCTA1751.2551162E-424.8570525
GTTATAG1500.001490453624.1757761
TTAGAGT3902.0008883E-1124.1665784
TATAGAC2252.6498305E-522.5554713
TAGAGTG4256.184564E-1122.176395
TATACTG3304.6838977E-821.9696185
GTATATG2003.09358E-421.7581961
Produced by FastQC (version 0.11.5)