FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1969-lungLR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1969-lungLR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937270
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG12980.1384873088864468No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC12210.13027196005419997No Hit
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG12080.12888495310849596No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC10690.11405464807366072No Hit
CCCTTGAAGTAGCCGTTCGGGATGGTCTCGATCCTGTTGCTGTCCAGGTA10040.10711961334514068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG2900.034.999785
GTAATAT2154.838512E-1033.736921
TTACCGC900.003619703732.23751
TATAACC2506.91216E-1131.9049053
AGCGAAA3850.030.1441561
GCTATAT2003.2404932E-729.0137481
CTATATA2456.427945E-826.6367472
TTACGAA1100.00966403126.3676932
ATACACG1100.00966403126.3676933
TAACCTA1801.5202508E-424.1665155
GCGAAAG4900.023.6771092
TATAATA4700.023.1418572
TATTGGG2603.1995332E-622.3111232
TATATAC3604.838512E-922.1561853
GTATTAT5900.022.129131
TTATAAT4307.094059E-1121.9289971
TAATATA3406.419941E-821.326812
ATAACCT3757.752533E-921.2688034
GTATACA2755.1887437E-621.1009081
GTATTAC3106.214723E-721.0583651
Produced by FastQC (version 0.11.5)