FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1969-lungLL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1969-lungLL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110071
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14230.12818999865774353No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT12530.11287566290804822No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC12240.11026321739780608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1600.049.863391
TATAGCG450.00963470748.339314
TTACGTG2151.2732926E-1137.099282
ATCGACA800.00203682636.249584145
AATACCG850.002741058134.1203275
ACGTCCG1102.436486E-432.9541669
TACGTGT2802.6193447E-1028.4856643
TATACTG4900.028.1144545
GACGACT1050.007704160627.6224634
GTGATCG1050.007709243327.6187299
GTTGACA10650.023.1546611
GCAATAC1600.002173736722.6590543
ATACCGC1650.002599851221.9714246
ACCGATC1650.002601211721.9694448
ACATGGT11650.021.7828275
GTATAAG3706.6229404E-921.5625481
CGTGTTG4058.058123E-1021.4831715
TTGACAT11600.021.2535842
TACTACC2404.3592816E-521.1484474
GACTTAA5850.021.076681
Produced by FastQC (version 0.11.5)