FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1969-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1969-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473533
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG69371.464945420910475No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT67321.421653823492766No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG63111.3327476648934709No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA61091.2900896030477285No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG53411.1279044966243112No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA51851.0949606468820547No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT48561.0254829124897316No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT46450.9809242439280895No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT39940.8434470248113647No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC35550.7507396527802708No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA35240.7441931185366173No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC29760.6284672873907415No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA28340.5984799369843284No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG27820.5874986537369096No Hit
GTATAAGGGAATGTGGTACTTATTGCATTTTGCGCTGGCACTTTTAAGAA24110.5091514213370557No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG23880.5042943152853128No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA21550.4550897191959166No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT21230.4483320064282743No Hit
TCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATA18610.39300323314320224No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT18300.3864566988995487No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG18170.383711378087694No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG18160.3835001995637052No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT17730.37441952303218573No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG17440.3682953458365098No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG17050.3600593834009457No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT16830.3554134558731915No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC16340.34506570819773913No Hit
ATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTT16340.34506570819773913No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG15930.3364073887141973No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG15760.33281735380638733No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC15760.33281735380638733No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT15270.3224696061309349No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA15210.32120253498700196No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC15120.31930192827110254No Hit
TATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGA15100.3188795712231249No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA14410.304308253067896No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA14270.3013517537320525No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT14160.2990287899681754No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG14040.2964946476803095No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT13960.29480521948839894No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAA13280.2804450798571589No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA13220.2791780087132259No Hit
TCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCA12440.2627060838420976No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC12420.26228372679411993No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC12390.2616501912221535No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC12220.2580601563143435No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT11890.2510912650227123No Hit
GTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAA11820.2496130153547905No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT11570.24433355225506986No Hit
GGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGA11320.23905408915534926No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTC11270.23799819653540513No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA11030.23292991195967336No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA10870.22955105557585215No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG10530.22237098576023212No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA10480.22131509314028802No Hit
CTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGA10420.22004802199635506No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT10280.21709152266051152No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA10220.21582445151657856No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG10180.21497973742062326No Hit
GGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCG10130.21392384480067914No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA10120.21371266627669033No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA9990.21096734546483562No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA9980.21075616694084678No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA9670.20420963269719322No Hit
GAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAAT9590.20252020450528263No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG9450.19956370516943908No Hit
CACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAA9450.19956370516943908No Hit
GAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTGACAAA9240.19512895616567377No Hit
ATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCA9230.19491777764168494No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACT9040.1909053856858973No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA8990.18984949306595317No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT8920.1883712433980314No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA8860.18710417225409845No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT8710.18393649439426607No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA8700.18372531587027727No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG8660.18288060177432194No Hit
ACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTTAA8610.18182470915437784No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA8510.1797129239144896No Hit
TTACCAACACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCT8440.1782346742465678No Hit
TTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAA8410.17760113867460137No Hit
ATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATA8360.17654524605465724No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG8280.17485581786274662No Hit
GTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT8250.17422228229078016No Hit
ATCTTACGAAAGGGGAGAAAGCTAATGTATTGATAGGGCAAGGAGACGTA8250.17422228229078016No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG8190.1729552111468472No Hit
CGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAA8060.1702098903349925No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAT8060.1702098903349925No Hit
ACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATT8060.1702098903349925No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT7990.16873164066707072No Hit
ATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA7870.16619749837920483No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG7860.165986319855216No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA7850.16577514133122717No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC7790.16450807018729424No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA7700.16260746347139482No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG7700.16260746347139482No Hit
CAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCT7530.1590174285635848No Hit
TAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTG7440.15711682184768538No Hit
AACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAG7400.15627210775173006No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTG7350.15521621513178596No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT7340.15500503660779713No Hit
CATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTTAAT7110.15014793055605416No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG7110.15014793055605416No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT7000.1478249667921771No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT6950.14676907417223298No Hit
GTTCATTGATGCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGG6820.14402375336037826No Hit
TCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATC6770.14296786074043413No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC6770.14296786074043413No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC6730.14212314664447884No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA6680.1410672540245347No Hit
ATCTCGCACTCGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCA6630.1400113614045906No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA6560.13853311173666882No Hit
CCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAG6500.13726604059273587No Hit
TCTTAATTATGGCCATATGATCCACAGTGGTTTTTGTCAGTATCTCGCGA6470.13663250502076943No Hit
GAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAA6390.1349430768288588No Hit
GATTAAGAAAGAAGAATTTGCTGAGATCTTGAAGATCTGTTCCACCATTG6330.13367600568492585No Hit
ATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAAT6310.13325364863694822No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC6270.13240893454099292No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG6270.13240893454099292No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA6250.13198657749301526No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT6150.129874792253127No Hit
CTGTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAG5920.12501768620138407No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG5890.1243841506294176No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG5890.1243841506294176No Hit
GCTGTTCTGTTGACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCT5830.12311707948548464No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATA5830.12311707948548464No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT5790.12227236538952933No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC5720.12079411572160759No Hit
CTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCT5720.12079411572160759No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATC5700.12037175867362992No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG5680.11994940162565228No Hit
CTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTC5660.11952704457767463No Hit
GCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA5650.1193158660536858No Hit
ATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGC5550.11720408081379756No Hit
TGTATTGATAGGGCAAGGAGACGTAGTGTTGGTAATGAAACGGAAACGGG5530.11678172376581991No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC5500.11614818819385343No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC5470.11551465262188695No Hit
GCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGC5420.11445876000194283No Hit
GATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAAT5400.11403640295396521No Hit
GTATTGAGAGGATTTCTAATCCTCGGCAAAGAAGACAAAAGATATGGACC5390.11382522442997638No Hit
AGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACT5330.11255815328604342No Hit
CTCCAGTATAAGGGAATGTGGTACTTATTGCATTTTGCGCTGGCACTTTT5330.11255815328604342No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG5310.11213579623806577No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGT5290.11171343919008812No Hit
CTTCTAAGTGTGTGCATATTGCAGCGAACTTATTTGTTTCAATTTTCGGG5220.11023518952216636No Hit
ACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGC5180.10939047542621105No Hit
ATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGA5150.10875693985424459No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT5140.10854576133025576No Hit
TCGTAAGATTGCTCAGTTCATTGATGCTTAATGCTGGTCCATATCTTTTG5140.10854576133025576No Hit
CCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACA5110.10791222575828928No Hit
GATATGGACCAGCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGG5100.10770104723430045No Hit
TACCAACACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTT5050.10664515461435635No Hit
ACACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTA5050.10664515461435635No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTATATGGGGGATCTCCAGTATAAG5030.1062227975663787No Hit
ATATGGGGGATCTCCAGTATAAGGGAATGTGGTACTTATTGCATTTTGCG4940.10432219085047927No Hit
GATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCC4930.10411101232649043No Hit
GGGCTATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTT4850.10242158413457986No Hit
GCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAGGCGAATCAATA4830.10199922708660221No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA4810.10157687003862455No Hit
GTATTGATAGGGCAAGGAGACGTAGTGTTGGTAATGAAACGGAAACGGGA4800.10136569151463573No Hit
GATTGACTTCGAGTCTGGAAGGATTAAGAAAGAAGAATTTGCTGAGATCT4770.10073215594266925No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAAC100.007089691145.000021
TTACTGG100.007089691145.000024
TAGCCGG100.007089691145.00002145
GCAGGTA7500.0120.8333362
CAGGTAC7500.0119.866673
AGTAGAA11100.0118.873881
AGCAGGT7750.0116.935481
TCTGACC256.4613305E-6116.0145
GGTACTG8300.0108.313255
GCTGTAT553.6379788E-12105.4545441
GGTCAAA2100.0100.119051
ACTGATC9100.099.5879068
GTACTGA9200.097.7173846
CTGATCC9400.096.409589
AGCGAAA1000.094.251
TACTGAT9750.092.205137
GTCAAAT2400.087.6041642
TCAAATA2700.080.555563
GCAGGCA2450.076.938784
ACAAGGC9450.073.6507958
Produced by FastQC (version 0.11.5)