FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1968_lungUR_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1968_lungUR_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938890
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG9650.1027809434545048No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG9520.10139632970848556No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT9410.1002247334618539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1457.2759576E-1250.0237051
TTACGTG1457.2759576E-1250.010382
GTATACG1102.4292679E-432.970171
TATACTG2900.032.4998255
TACGTGT2257.912604E-1032.225483
CTGTCGT900.003628217432.2220469
AGCGAAA3102.7284841E-1128.0778181
GTGCGAA1606.8279565E-527.187354
TATAACC2954.820322E-1027.0366293
GTTATGT4050.026.8645821
AGTCGAA1100.00966151126.3691082
TATACGT1409.98204E-425.8982322
TAACCTA2101.5550031E-524.1665345
CCGACTA2451.911967E-623.6733384
GTATATG2202.2204642E-523.0791171
ATTATAC2202.2268763E-523.0705133
GTTGACA8500.023.0403291
CTAATAC1902.1915301E-422.8970493
CTATACT1600.002175121622.6561244
GTATTAT9650.022.5495451
Produced by FastQC (version 0.11.5)