FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1968_lungUL_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1968_lungUL_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG30.23828435266084197No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG30.23828435266084197No Hit
GGTTGGAGATGTTGAAGGAGTTCTTGGGGAGCCCCCGGTCTGACAGCCTG20.15885623510722796No Hit
GTATTAATTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTTTTT20.15885623510722796No Hit
GTCCAGAGGGCACACGTGGGCTTGCCACCAAGCACCCGGCCCTGAACTCG20.15885623510722796No Hit
CTTTAGAATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTT20.15885623510722796No Hit
GTTTACTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA20.15885623510722796No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC20.15885623510722796No Hit
CTCCAGGACCCCCCATTTCTGTAAATCACAGAATCTGGGCTTGAAGGGTG20.15885623510722796No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC20.15885623510722796No Hit
GAAGGCACACATCAGCTGTGACTCAGGAGCTCTGGGACTTGCTGGAGAAG20.15885623510722796No Hit
CATACAACCTACTAAGATTGAATTCTAAAGAAACAGACATCTGACTGTTC20.15885623510722796No Hit
CCTCTCTACACAGCAGATTTAAAAATACAGAGTGCTAAGGTTGGCACAGG20.15885623510722796No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG20.15885623510722796No Hit
GCCATATAGTTTTGATCCAAGTATTAGCTGCTTCTGCACCCTGGCCAAGT20.15885623510722796No Hit
GTATTAATTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT20.15885623510722796No Hit
GCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC20.15885623510722796No Hit
AAGTTAGGTTGCTTATTTGAGACGTTTCTTGTTTCTTGAGTTAGGCCTGT20.15885623510722796No Hit
CTACAAATCATCCACAGAAAAAAATGAATACCAATCCTTCTCCAACTCTG20.15885623510722796No Hit
GATACATATTTTTTAAAAAACACCAACAAGAATAACAGATCAGCAAAGAG20.15885623510722796No Hit
GACCAATGAGCAGTAGAGAGATTAAATAAGAAATCAAAAACTTCCAACAA20.15885623510722796No Hit
GTTCTTTTCTGTTTCCTTGAGGTATCAAGTTAGGTTGCTTATTTGAGATG20.15885623510722796No Hit
GAATTGGTGTCTTTGTGTTTTGTTTATTGCTGCACCCACAAGACATGGGC20.15885623510722796No Hit
CTTCCCTCAATGATCCCCCATCCAGACAGAAGATCACAAAGGAAACATCA20.15885623510722796No Hit
GGGGAGAGAAAAGAGGAGAGAGGAATGGAGTCAGCCCGTGAGGTGGGGGG20.15885623510722796No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT20.15885623510722796No Hit
GTCCAGGTGACACACCTGCAGGGAGGAGACACAGGAAGTTACTAGCCTGC20.15885623510722796No Hit
GGATCACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCAT20.15885623510722796No Hit
GGGTAAGGATTTTTATGTTTACGTTCATCACAGATACTAGTCTGTAATTT20.15885623510722796No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTTT400.007091208418.12499835-39
Produced by FastQC (version 0.11.5)