FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1968_lungLR_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1968_lungLR_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACATTAGTTTGAT50.282326369282891No Hit
GCATGAGGTTCTTGAGGCCCTGGAAGGTGTCGGGCTTGAAGACACCATCG30.16939582156973462No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC30.16939582156973462No Hit
CAGTAAAACAGGTTCTAAAATGGGGCACCCGATTAGTCGATTAGTCTAGT20.1129305477131564No Hit
TTTCAATGGGCTGGCCCTGTGAAAAGCCTGGGCTGTAATGATTAGGAAGG20.1129305477131564No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG20.1129305477131564No Hit
ATAATGCTGAGTGAGCTGCACAGAGGTGGGACTCCTGCTGTCGCATCTAT20.1129305477131564No Hit
GTAAGTGGGGAGACCAAGCACTTGAGAGGCTAAGGGTCTTCTCCAGCAAG20.1129305477131564No Hit
GAGCATTCCTTCCTCTTGTTTGTCTGGCAGAGTTGGAGAAGGATTGGTAT20.1129305477131564No Hit
GACTTGAGGGTCGGGACCCCCAGCCAAAGACCTAGCCTGCTCTGGTGAGG20.1129305477131564No Hit
CATCATCAGAAATGAGAGAGATGTTCCCTGAAACCATTGATATGCAAAGG20.1129305477131564No Hit
CTTTTATACATATTCTCGTATTGGGAGTCAGGATATGGTCATTGGAGAAG20.1129305477131564No Hit
CTCCACAGAACTGGGGGCACAGTCTGAAAACAACTGAATTGTGGGATCTC20.1129305477131564No Hit
ACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCGGCCCTGCCCCGGCAC20.1129305477131564No Hit
CCTTCTGCCAGGATGGCTCACCCTCACCAGAGCAGGCTAGGTCTTTGGCT20.1129305477131564No Hit
TGTTGAAGGAGTTCTTGGGGAGCCCCCGGTCTGACAGCCTGTTGTAGTTC20.1129305477131564No Hit
TTACTAGCCTGCTGGACCTCTGGAGTTAGAGGCCCTGTCTTGGCACAAGA20.1129305477131564No Hit
GGATAATGATCATCTCAATGGACACAACTCCATGAGGAGCCAGTCCCCAT20.1129305477131564No Hit
GTACAGGCCGACAAGAGATGCTTTGACAGGTCTCTCCCACGGCCCCCCTG20.1129305477131564No Hit
CTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCAA20.1129305477131564No Hit
GGTCTGGGCCTGGGCCTGGGTCTTGGTCGCCTTGTCAGCTGGCCTTGGGC20.1129305477131564No Hit
TATGACCTAAGGTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTC20.1129305477131564No Hit
GTTCCTAGCACTGTGGGTATGCTGGCTGCACGCCCAGCGATGTGTGATTC20.1129305477131564No Hit
TCCCTAAACAAGGTTACTCATTAGCTGAGTGTGCCAGGGAGCGGGAACAG20.1129305477131564No Hit
CTACACAGCAGATTTAAAAATACAGAGTGCTAAGGTTGGCACAGGGGCGA20.1129305477131564No Hit
CCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGA20.1129305477131564No Hit
GGCCAAGATGAGGAGAAGTGGGAGGAGCCAGTAGAGGGATGACCTCATGG20.1129305477131564No Hit
CTTTAAGACCCAGTGAATGCTCACTAAATAATTCTGGAATAAATGAAAGC20.1129305477131564No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA20.1129305477131564No Hit
CATTAATTCCATTATCAGTTGTAGTGCCCTTCATTGGCTCTTGTTACAGT20.1129305477131564No Hit
AGTCCATTAGTCGGTGGAGCTCTGTGACTCTTGATCTTGTGGTTTTGAGG20.1129305477131564No Hit
CAACTGAATTGTGGGATCTCTAAGGACCCTTCCAGATGGACCGCTTAGGA20.1129305477131564No Hit
CAATAACAGATCAACAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACA20.1129305477131564No Hit
GTCCAGGACCCCATGGTTTCACCTGTGAATTCTACGAATCATCCACAGAA20.1129305477131564No Hit
CTATGCCCAGTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACA20.1129305477131564No Hit
CAAGTGGATCAAGCAGTGAGGGGCAGCAAGAGCCCTGTGGACCAAAGGTT20.1129305477131564No Hit
GTTCTAAAATGGGGCACCCGATTAGTCGATTAGTCTAGTCTATTAGTCAG20.1129305477131564No Hit
GTTTATGTTCATCACAGATACTTGGGAGGTCTCGATAAGGGCCGTGCTGG20.1129305477131564No Hit
ATCTCATTCCCTAAATAAAACTGTCTTTTGAGTAAACCTTCCACATATCA20.1129305477131564No Hit
TTTCTGTTTCCTTGAGGTATCAAGTTAGGTTGCTTATTTGAGATGTTTCT20.1129305477131564No Hit
GTCTTAGTGTCTCCCGCTCACCACAGGGTCAGAGGAAATTTAAACACACA20.1129305477131564No Hit
GTAGTAGAGCTAGTAAGTGGCTGTGCAAGAGGAAGAAGAGGAAAGCGTTG20.1129305477131564No Hit
CTTCTAACCCACTGAGAGTCAACAACTGCCATGTAAACTAACATCTCCCC20.1129305477131564No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)