FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1968_lungLL_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1968_lungLL_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834856
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11620.13918567992563985No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11600.13894611765382295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAC400.00604061654.397561
GTTACGT700.001054386741.445761
GCTACTA1251.26487685E-534.7998439
ATAGTAT1051.8535042E-434.523656
TATACTG2051.1381417E-831.8291245
TTACGTG1153.15952E-431.5253722
GATATGT4100.030.0734481
GTGCGAA1952.6051748E-729.7452354
TAGGTAG1358.081803E-426.851735
GTGTTAC5750.026.4892461
GTTAATA1701.0281901E-425.598851
TATAATA2859.8789315E-925.4415282
TAGTACC2859.884388E-925.4400044
CCGACTA2001.0647367E-525.3764044
TTATACT1751.2550112E-424.8585194
ATAGTGA3652.2009772E-1023.8355086
GTATAGT1550.001803881123.3968011
TTAGACT1550.001807657523.3883914
TATAGTG3753.074092E-1023.1998945
TAGTGAT3503.4997356E-922.785617
Produced by FastQC (version 0.11.5)