FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1968-lungUR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1968-lungUR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843155
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11740.13923892997135756No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11700.13876452135135298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCG752.5503394E-548.333015145
TATATAG1351.024091E-842.9703252
CTACATC4100.042.4462973
TATAACC2300.037.832573
GCTACTA1157.141698E-637.825849
GTGCGAA1801.2907185E-732.2258344
TAACCTA2151.8098945E-830.3486395
AACCGCG2054.03812E-728.2925017
GTAATAT2354.276444E-827.7789541
GATCCGC1050.007708024727.6188666
ATATAAC1855.8069727E-627.4371342
GTTATGT3204.1836756E-1127.2002261
TAATATA2156.113514E-726.9813672
ATAACCT3005.857146E-1026.5863114
TACTATA1100.00966266726.3681552
GTATTAT5600.025.9049781
ATAGTAG1409.993667E-425.8926895
GTATATA2259.067571E-725.7898431
GTTGACA9100.025.506441
TATACTG2601.1443808E-725.0959915
Produced by FastQC (version 0.11.5)