FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1968-lungUL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1968-lungUL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069435
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT18780.17560674561801326No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG18160.16980929182231738No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC13740.12847905669816306No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG11390.10650483666609004No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG10960.10248402193681712No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA10840.10136193410539208No Hit
CTTTAATACCTCACACTTCTCTCAATGATCCCCCATCCAGACAGAAGATC10790.100894397508965No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC10790.100894397508965No Hit
CTTCCCGTATTGTAGAATTTGGTTTGCAGATATTTGGGTGAGGATTTTTA10780.10080089018967958No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCG657.33715E-444.614967145
GTTACGT1151.4071156E-744.152731
CTACGGG855.3376185E-542.6526454
TTACGTG1252.7104034E-740.6167072
AGTCGAA1051.8493237E-434.538022
GCGTTAA1751.0093936E-733.1425489
TTTTGAC4550.031.881252
TCTACGG1153.158952E-431.527343
TTAGGTA1153.1598183E-431.5258664
GTTATGT5400.030.8952311
CTATTAC950.00472333430.531743
TGCGTTA1902.0769949E-730.526038
GTATATC2401.5734258E-930.2235951
TATAGGT1204.0484584E-430.2207682
TATACTG2401.5807018E-930.2094655
TAACCTA3700.029.3929925
GTAGTAG1000.0060596729.014651
GTTAATA2054.0217856E-728.3069761
GTATTAT13500.027.9400351
AGCGAAA4450.027.7106231
Produced by FastQC (version 0.11.5)