FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1968-lungMR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1968-lungMR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555158
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG23960.1540679467938306No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG21270.13677066896096732No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT20900.13439148948209764No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC18710.12030931905311229No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC17690.11375049994920129No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA16090.10346215625679192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1152.4683686E-950.4743731
TACGGAT800.002034586436.2586173
GTTATGT10050.035.3760031
TTACGTG1751.006847E-733.1539342
GTTGACA16150.030.9995441
GGTACGG1453.472979E-530.0235461
GTGCGAA2502.4447218E-929.0040994
CCGTACG200.00607753428.99943485-89
GCGATTA2303.4910045E-828.36901145
CTACTGA26750.028.1872716
ACATGGT17500.028.172695
CCGACTA7150.027.3814894
TTGATTA10100.027.276697145
CATTCTA17950.027.0703282
TACTGAA27750.026.6481297
GTACGGA1100.0096574626.372452
TACACGT1100.00967109426.3648175
TGTGTTG14400.026.181735
GTATATC3357.4578566E-1125.9905341
TATGTGT14850.025.881573
Produced by FastQC (version 0.11.5)