FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1968-lungLR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1968-lungLR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105208
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT16360.14802643484303407No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG15420.1395212484889722No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC13350.12079174236885727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT300.001935849272.4995049
GTTACGT1154.0017767E-1156.766991
TTACGTG1402.728484E-1046.6173672
AACCTCG1051.8539169E-434.523579
CCGACTA4200.031.0740284
GTATATC2401.5734258E-930.2231661
TATACTG5050.030.1496735
TAGTACC4450.029.3256429
TCATAAG6500.029.0037363
GTTATAG1255.150302E-429.0037363
GTATTAT14200.027.0731451
CATAAGA6200.026.8974114
CCACCGA5150.026.7607061
GTTATGT4400.026.3765831
TTTTGAC5150.025.3453642
CACCGAC5250.024.8625982
TAGTCGG2351.3307308E-624.6806818
GCTCATA7350.024.6719721
TACGTGT2951.4262696E-824.5794373
GTTGACA9700.023.9292721
Produced by FastQC (version 0.11.5)