FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1968-lungLL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1968-lungLL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948329
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG9740.10270697194749924No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG9540.10059799921757111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACG450.0096332848.3405764
GTTGACA7550.030.7444291
CCGACTA3100.030.4077824
GTTACGT1000.00605891629.0150551
GCATACG1000.00605891629.0150551
TACTGAA11050.026.2441257
CTAATAC2507.8145604E-826.1052883
AGCGAAA5100.025.601521
ACATGGT9100.024.6975985
CTACTGA11550.024.4803146
GTCTAAT1801.5148609E-424.1792131
GTAGTAG1902.1853572E-422.9066241
GTAGGCT3503.5015546E-922.7855246
GCGAAAG5750.022.7026442
CGAAAGC5750.022.7002493
TATAACC2903.279274E-722.5045573
GTACAAT4607.2759576E-1222.0766741
CTATAGT1650.002598499921.9729884
TAACCTA1650.002600887821.9695155
TAGTCGG1650.002600887821.9695158
Produced by FastQC (version 0.11.5)