FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1967_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1967_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3317
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACT60.18088634308109736No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC50.1507386192342478No Hit
CTCTGGGAAAGATCCATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATT40.12059089538739826No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT40.12059089538739826No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC40.12059089538739826No Hit
ACATAATACAGACTAGAACTAAAATTGGATTCCAGTTTGACCCTAAAGGC40.12059089538739826No Hit
CTTTATGCCTCTGCAGTGGTAGGACGGTTTTTCATGGAGGAAGGGGCCCA40.12059089538739826No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG40.12059089538739826No Hit
CGTTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGT40.12059089538739826No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA40.12059089538739826No Hit
ATCATGACCTGAGCCAAAGGCAAAGGCTTTAACCCACTGAGCCACCCAGA40.12059089538739826No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)