FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1967_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1967_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTGAAGTAGCCGTTCGGGATGGTCTCGATCCTGTTGCTGTCCAGGTA50.22522522522522523No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG40.18018018018018017No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC40.18018018018018017No Hit
TCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACT40.18018018018018017No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT40.18018018018018017No Hit
CTCCAGGACCCCCCATTTCTGTAAATCACAGAATCTGGGCTTGAAGGGTG30.13513513513513514No Hit
ATGTAGGCCATTTCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAAT30.13513513513513514No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.13513513513513514No Hit
CCCTAAACAAGGTTACTCATTAGCTGAGTGTGCCAGGGAGCGGGAACAGT30.13513513513513514No Hit
GGTGACACACCTGCAGGGAGGAGACACAGGAAGTTACTAGCCTGCTGGAC30.13513513513513514No Hit
GCTGTGATGTGCAAGTGTTTTCTCTCGAGTGAATAAACATTGAGTGACTA30.13513513513513514No Hit
GCTTAGGACCCTATGGCTCATGTCTCCTAACATCCCTCTTCTAGGAACAG30.13513513513513514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG350.003436902520.714287120-124
Produced by FastQC (version 0.11.5)