FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1967_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1967_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC50.2679528403001072No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG40.21436227224008575No Hit
TCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACT40.21436227224008575No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC40.21436227224008575No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC40.21436227224008575No Hit
GAACAGACTAATGACCAGTAGAGAGATTAAATAAGAAATCAAAAACTTCC30.1607717041800643No Hit
AGATGATGTCACTGAATGGTCTTCGCGTGTGCTCACTTCTGTACCTTTAC30.1607717041800643No Hit
CCTTTAGGTGGCCAGTCAGGTGCTGTGTCCCACATACCCGATCAGGCAGT30.1607717041800643No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG30.1607717041800643No Hit
GTTCATGGGCAAACCAAAATAGGAACACAGAAATACTTTGAGGTAAATGA30.1607717041800643No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG30.1607717041800643No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC30.1607717041800643No Hit
GAAGTAGGGGCAGAGGGAAGGTGGAAGAGGGAAAGGGGAGAGAAAAGAGG30.1607717041800643No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG30.1607717041800643No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA30.1607717041800643No Hit
CTCCACAGAGCAGGGACTCCAACGTGGGGCTCGATCCCAGGACCCTGGGA20.10718113612004287No Hit
CACATATCCTGACGCTCGTTTCACATCAGCACATAGAATCCCCTCACTCC20.10718113612004287No Hit
CTGTAACAAGAGCCAATGAAGGGCACTACAACTGATAATGGAATTAATGC20.10718113612004287No Hit
ACCTCCGACCTTTCAGAAGATTCTGAACTTCGGGAGGCAACGCAGTCTCT20.10718113612004287No Hit
CAGAAAGATATATATTAAAAATAAAAACACCAACAAGAATAACAGATCAA20.10718113612004287No Hit
GTAGCATTTGGTTTGCAGATACTTGGGTGAGGATTTTTATGTTTATGTTC20.10718113612004287No Hit
GATATGTTCCTTCTATTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCC20.10718113612004287No Hit
CTCCAGGACCCCCCATTTCTGTAAATCACAGAATCTGGGCTTGAAGGGTG20.10718113612004287No Hit
CTCTGAAACTCTGTTTCCATATCTCTAAAGTTATGATAATGATCCTCACG20.10718113612004287No Hit
TCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAGGAGGGAATGGAT20.10718113612004287No Hit
ATACTACTCTTGCCCTCATTTGTGGACGTGCACAAAGTGGTGAGAAATTT20.10718113612004287No Hit
GATACATATTTAAAAAAAAACACCAACAAGAATAACAGATCAACAAAGAG20.10718113612004287No Hit
ACCTTATTTAGTTTGTAAACATAATTCAGAGTCAGTGTGGCTGTGTTTTA20.10718113612004287No Hit
CTCCAATACGAGGATGTGTATAAAAGTGCTTTATAAACTAGGAGATACTG20.10718113612004287No Hit
ACCTGTTACTTAGATGGACTTGACAGATACACACAGAACCCTCCATCCAA20.10718113612004287No Hit
TGCTGGCTGCACGCCCAGCGATGTGTGATTCTGTCTCTCTTCTTCCAAGG20.10718113612004287No Hit
GAATGGAGTCAGCCCGTGAGGTGGGGGGATTGCACTCTGGGAAAGATCCA20.10718113612004287No Hit
ATATATATCTTTCTGTTGTCTTTTTAGTTTGTATTTCATTTATTTAAGTT20.10718113612004287No Hit
AAACAAGGTTACTCATTAGCTGAGTGTGCCAGGGAGCGGGAACAGTGTGG20.10718113612004287No Hit
GTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACATTAGTTTGAT20.10718113612004287No Hit
ACCCCACACTGGGCATAGAGCTCACTTTATTTTTTTAAAGATGCCATTTA20.10718113612004287No Hit
TAGTCGATTAGTCTAGTCCATTAGTCGGTGGAGCTCTGTGACTCTTGATC20.10718113612004287No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCACAT20.10718113612004287No Hit
GGGATAAAGCAACCTTGCTGTGTCAATGAGGAAAAGGGAGTTTCAGTAGC20.10718113612004287No Hit
GACTTAAATCAACCATATTTTCCAACCACAGTGGTATAGAACTAGAAATC20.10718113612004287No Hit
ATCTGGAGGTCTTCTGGTCTTGAACTGGACTCCCTGCATCTGTTCTTTAT20.10718113612004287No Hit
ATCCTGGCAGAAGGCACACATCAGCTGTGACTCAGGAGCTCTGGGACTTG20.10718113612004287No Hit
CAGAAATACTTTGAGGTAAATGAAAAATTAATACAATGAAACAAAATTCA20.10718113612004287No Hit
TTTCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCA20.10718113612004287No Hit
CATGTATTTATTGTAGAGACAGAAAGAGAGTGCACAAGCATATGCTAGAG20.10718113612004287No Hit
GCCAAAGACCTAGCCTGCTCTGGTGAGGGTGAGCCATCCTGGCAGAAGGC20.10718113612004287No Hit
CTCTATCAGCTTTGTTCTTTTCAAAGAACCAGCTCTTTGTTGATCTGTTA20.10718113612004287No Hit
CTAAAAAGACAACAGAAAGATATATATTAAAAATAAAAACACCAACAAGA20.10718113612004287No Hit
GTTCCCTACTTTAGTGAAGCTTTCTCTAACAAGCCTATCTAAAATATCTC20.10718113612004287No Hit
CCAATGACCATATCCTGACTCCAATACGAGGATGTGTATAAAAGTGCTTT20.10718113612004287No Hit
GATCTAAGACGTTGTCAGCCCACTGTGAATCCTGCAGTGGTTTCCCGTGA20.10718113612004287No Hit
GTACAGTCTAGCTAGGGGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAG20.10718113612004287No Hit
TCCCATAGGTGACCTTCCTTTCACAAGAGCAGAATCATATGGGCTAGCTA20.10718113612004287No Hit
GTTGCATCATTTTACTGCATTAATACCATTATCAGTTGTAGTGCCCTTCA20.10718113612004287No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC20.10718113612004287No Hit
GTACAAAAACTTATTGACATAAGTTCCAGGTGAGCAAAAGAAAAAAAAAT20.10718113612004287No Hit
GTCTTGGCACAAGAGTTGGCCAGGATGGGCTCCAGGGGGGCCGTGGGAGA20.10718113612004287No Hit
GAGGAATGGAGTCAGCCCGTGAGGTGGGGGGATTGCACTCTGGGAAAGAT20.10718113612004287No Hit
AGGTGGAAGAGGGAAAGGGGAGAGAAAAGAGGAGAGAGGAATGGAGTCAG20.10718113612004287No Hit
GGTGTTGACTGTGCAAAGAGAAGGAGAGTTTTGAATGGGACTTTTGTTAG20.10718113612004287No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA20.10718113612004287No Hit
GTTCCTGCACCTTGCCCCTGAGTGCCTCCCAGACCTGAAGTTTCCACCCT20.10718113612004287No Hit
GATAAAGGCTGCACAAGCTCAGATTTCTCTTCTCTGTGCAATGGGCTTTT20.10718113612004287No Hit
GAGGCTGATGTTTCCGTTGTGATCTTCTGTCTGGATGGGGGATCATTGAG20.10718113612004287No Hit
CCCTCACCCTGTGAGGCGTGTGTCCCTGAGACGTGGGGGGATGTCAGGCA20.10718113612004287No Hit
ACTGTAAATTCATAAACACAGAGACTAAACAACATGCTACTGAACAACCA20.10718113612004287No Hit
CTCTAAGGGCTGCCTGTGTGGCTTGGTCTGTTAGGTGTTGGCCTTTGGCT20.10718113612004287No Hit
GTGGGTGGGTGAGGCTGGAGGGAAGAAGGCAGGGAGCGGGAGTGGTAGGT20.10718113612004287No Hit
CCTCCATAGGCTTCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAG20.10718113612004287No Hit
ATACTAACGTATGCTTCCTAGAATGAGAAGGAAGAAAATGAATACAGAAG20.10718113612004287No Hit
AGTATATAGTGATACAGGCCTAACTCAAGAAATAAGAAACATCTCAAAGA20.10718113612004287No Hit
GTGTTCAGTAAGTGGCACCCATGCTTTTTCCAGCTCCATGGCCAAGGAAA20.10718113612004287No Hit
ACTGTACACAGCTCATCTCGGCAAAATGATTTACTCAGGCTGCACACATT20.10718113612004287No Hit
GCCTAGGATGGCACTCTGCTCACAATGCCATCTCTCGGCGTAGGGACGGC20.10718113612004287No Hit
CTATTGAACAGATCCAGCATGATGCCTGTGCTTGCCTCTTTACTATCTTT20.10718113612004287No Hit
GATTTTGTATCCTGAACACCCAGCATCGTGTAGCATTTGACATATAGAAA20.10718113612004287No Hit
GGGGAGAGAAAAGAGGAGAGAGGAATGGAGTCAGCCCGTGAGGTGGGGGG20.10718113612004287No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA20.10718113612004287No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)