FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1967-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1967-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542447
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACTGTGCTGCTTCTGAGAAACCAGCAGGGCCTGTTAATTCTTT11820.21790147240191207No Hit
CTTATAAGGGGTCGAGTATTCATTACCACAATTCAACAACAAAAAGACAA8940.16480872785728373No Hit
CTTTTATAATATGCAATGTGAGGTCATCGCTGTTCACTAGAAAGTGGAGA7510.1384467053924162No Hit
GTGTAAGAGAGTGTGTCTAGTTTCATTCTTCTATATATAGTCACCCAATT7030.12959791463497816No Hit
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT6750.12443612002647263No Hit
GCATTATCCAGCTCCAGAATTTTAGCCACGACTCCCTGTTTCAGGTGACT6660.12277697175945299No Hit
TCCCTATACTGTCTCTTTCTGGATGTTCAGACACAGTGATCTATCTGAGG6560.12093347368498675No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT6100.11245338254244194No Hit
GAGTAGCATTATCCAGCTCCAGAATTTTAGCCACGACTCCCTGTTTCAGG5580.10286719255521738No Hit
GGTCTACATCTCCTCCAGGCTATATCTTCTACGGGATCTCAGACAAGGAC5490.10120804428819774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACC100.007090177144.99861145
GACGCGC300.001935336372.499306145
TAGTTAC400.006043011354.3895233
GGGTCGA3700.052.90499
CCCTATA2900.050.0133552
GGCGTAA450.00961960248.355161
ATACTGA2500.043.4995846
TATACTG10150.042.8606835
TGATCCG855.3385484E-542.6466529
CCTATAC5450.042.5801773
TACACCG1755.2750693E-1141.4319955
CACCATA3050.040.409457
TACTGAG3450.039.9271557
GGGGTCG5000.039.1496288
GGTAGTA1303.6944766E-739.0380868
CCGGGCG750.001482856738.666298145
AGGGGTC5100.038.381997
TAAGACT951.0266529E-438.1680874
CTTATAA5350.037.961061
TATAAGG5350.037.954063