FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1967-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1967-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853422
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATATGCATGACCTTGTTTAGCCTTGCAATGGCCTTGTAGAGTTG19440.227788831316746No Hit
GTCCTGGAGTGACCCAAGACAGGGATGGGAAACAGTGGGTGACCACACAT12840.1504531169808137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3900.065.075122
CTTATAG2350.058.6235123
ATATACG1353.6379788E-1253.7096524
CGAAAGC4750.051.9004823
TATATAC1550.051.457313
AGCGAAA4950.049.821011
TATACGG1402.728484E-1046.609575
TCAATAG2950.044.2421883
TATAGAT2650.041.0446852
CGCTTAC757.517487E-538.6845471
ATACGGG1803.1996024E-936.2497636
CCTATAT2252.0008883E-1135.460841
TATACTG1458.692896E-735.0018235
GTATATA2501.8189894E-1234.8160931
AATAGAG4800.034.7413945
CTATATA2404.3655746E-1133.2347952
GTACTAA1102.4359445E-432.954336
ATGCCCG2301.0022632E-931.521536145
GTGATCG1851.6432386E-731.3511499
CGTTACT2101.43281795E-831.0857981
Produced by FastQC (version 0.11.5)