FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1967-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1967-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705555
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12710.18014187412745994No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11500.1629922543246097No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA9460.1340788457313746No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA8140.1153701695828107No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG8130.11522843718774582No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT7730.1095591413851507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1203.608875E-948.3468481
GTACCGA800.002036248636.249867
GTAGCAT13900.034.434091
GCATGTT12350.034.050864
GTTGACA6500.032.3551981
AGTACCG1255.1524397E-428.9998876
TAGCATG14350.028.8018782
CATGTTG10950.028.470215
GTATTAT7200.028.2023281
GAATAAC5400.028.2023281
ATGTTGT15550.027.9741676
GTATACG1050.007696428327.626771
TACCGAC1050.00770708527.6189388
TATACTG3700.027.4323255
TTGTTTA16200.026.4042199
AGCATGT15800.026.1568153
TTGACAT8500.024.7387052
ACATGGT8800.024.7158135
CGTGTTG2451.9108174E-623.6733785
GTTATAT2502.2721088E-623.2064861
Produced by FastQC (version 0.11.5)