FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1967-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1967-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471739
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC6170.13079266289198052No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG5630.11934565511861432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2100.051.8291361
GCGAAAG2450.047.3665352
TTACGTG657.3176913E-444.633852
CGAAAGC3150.036.8289223
GTTACGT900.003611447832.2492371
TACGTGT900.00362460332.2253073
TCGACTG1153.1597755E-431.5214185
TATATAG1952.6025918E-729.7432845
TATTAAC1753.7569262E-629.0120052
TATAGTG2802.6193447E-1028.4818525
GCCCGTA1050.00770477527.6187659
GTATTAT5700.026.7329241
GCAGTGC3950.025.7177491
CAACCCG2301.1011471E-625.217134145
TATAGAC1450.001224798525.0023943
CCTATCC1450.001224798525.0023943
CAGTGCA4900.023.6832682
CAACCGG2451.9086874E-623.673227145
GTTATGT2852.750021E-722.9139351
ATATTAA2953.8343205E-722.137191
Produced by FastQC (version 0.11.5)