FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1966_lungUL_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1966_lungUL_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872121
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG10350.11867619286773282No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC10130.11615360712561676No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG8850.1014767446260324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT701.6929698E-551.8003581
ATAGCGT657.3362066E-444.6152086
TATATGG1502.5976988E-838.668733
GATATAC1157.1390405E-637.8281063
GTATTAT7150.034.4852751
GCGTTAA900.00362804932.2220959
ACCGTTC900.00362804932.2220958
TATACTG2807.2759576E-1231.0730865
TATTAGG3201.8189894E-1229.4563872
TTACGTG1255.149968E-429.003212
TTATAGA2553.0213414E-928.4328924
GGATATA1555.4954802E-528.0676232
TATAAGC1050.00770591227.6205224
TGCGTTA1050.007708068527.6189388
TAAACTG3108.58563E-1025.7271795
ACATGGT7350.024.661185
GTATATA3301.7662387E-924.17351
GTTGACA7300.023.8423561
GTGCTAA14350.023.752361
TATAGAG2751.9670006E-723.7285395
Produced by FastQC (version 0.11.5)