FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1966_lungLR_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1966_lungLR_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGGTTGTAGGAATTTATAACCACACAAAGCAGTAGAGTCAGCAGG10.8928571428571428No Hit
GAGGGCAGGTGGAAGCGGGAAAGGGGAGAGAAAAGAGGAGAGAGGAATGG10.8928571428571428No Hit
GTGCATGTAGCCCATGAGTTGTTACCAGAAATTCTCAAGGTTGAACACGG10.8928571428571428No Hit
GATAAGGTGGGGTGGGGGGGGTTCGTTGCTCTGGGTGTGCTGGGAGGGTG10.8928571428571428No Hit
GTATTCACTATTCCTAGTCCTTAAAGATTATCTCATGAAGATTGTGTTTT10.8928571428571428No Hit
CTTCAGGCTTGCACCTGCCGCCAACACAGCATGCCGCCCGTGGTGAGCAT10.8928571428571428No Hit
CCCTTTATCTCTGATGTGGGAGTGCCTGCCGAGGCCAGGATGTCGCTCAG10.8928571428571428No Hit
CCGTGGGCTTGCCACCAAGCACCCGGCCCTGAACTCGCAGAAACCCCTCC10.8928571428571428No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC10.8928571428571428No Hit
CCCGCCTCCCTCTGCTCCAGGGCAAAGGGGCACCAAGAGAGGGAGAATAG10.8928571428571428No Hit
GCTTTTGTGTTCAGTCCAAAAATAAGATCACATGAGAGGGGAGAAATAAA10.8928571428571428No Hit
TCTCATGGCTTATCTCCCTCTCTGATTTCTTCCCCTTCAGTTTCCCCTCC10.8928571428571428No Hit
TTTATATTCTAAAAAGTAAAAAACAAAACAAAACAAAAAACCAAAACAAA10.8928571428571428No Hit
CTCCTACAGATACCCTGTGCATTCACATTGTGTTGCCCTGTAAGACATAC10.8928571428571428No Hit
GGTGGAAGAGGGAAAGGGGAGAGAAAAGAGGAGAGAGGAATGGAGTCATC10.8928571428571428No Hit
ACTAAACACCACCAAGGTGGTGATCAATCTGTCAGCGTGTTGTCTTCAGG10.8928571428571428No Hit
TCTGAGTTCAGAGCCCAAGTACTTGGCACTGCTGAAGCCACATCTCTCCT10.8928571428571428No Hit
AACCCGAGGGGAACGCTACTAGCTGTTTTGCTAACACAGTGGGCATAAAA10.8928571428571428No Hit
GTAGGGAGAAATGAGGTTTGTTATACAGTCTGAAGCAGAAGCAAAGAGAA10.8928571428571428No Hit
GTGTGAAGTAGCCAGCTGCAGGCATGGGATACAGTCAGGACTCTAGACTG10.8928571428571428No Hit
GAGTACATACGACGGGGGTGAGGGCTCAGGGCTATTGTGTGGTGTTGCAG10.8928571428571428No Hit
GTTATGCCTTGAGGGTGGTGGTGGGGAAAATCTGAAAAATGCAGCCTCTT10.8928571428571428No Hit
CAATGATCCAGTCCTCTGGGCGGTCATGAAGATTGACCCAGCACTCAGTA10.8928571428571428No Hit
GCCACTTACTAGCTCTACTACCGCTCTCCCAACCTGCCAGGAACATACTG10.8928571428571428No Hit
GATCTAGGCTAAAGCAGGCAGGAACAGCAGCCCGTAGCACCGACGCTGAT10.8928571428571428No Hit
GTGTGTGTATGTGTGTTCCAGTCCAGGTTTGTCAAGCTTCATATTCTCTT10.8928571428571428No Hit
ATATTAAACAAAACAGTCGTGGTGGTTTGGTCTAACCAGAGTTTTTAATT10.8928571428571428No Hit
GGCAATAAGCAGCTAGATCAACACATCAAAACTACATAGCAACCATGGTT10.8928571428571428No Hit
ATTTTAGGCTGTGGGCTATATGGTCTGTGGTGCAATTATTCAACTCTGCA10.8928571428571428No Hit
GCCCCCCCGGAGCTCCGGGCACCCGACACCTGTCTGGATAGAACAGGAAC10.8928571428571428No Hit
AGATAAGAAACTATCCAGAAGGTGCCACGTACAAATCAGAGGAGTTCCAG10.8928571428571428No Hit
GACTGAGTGGGCCTCGGGCTCCCGGCCCCGTGGCTCGCTCACCCGGCCTT10.8928571428571428No Hit
GTTGGGGGACAGTCCTCGAGCTGCTGTAACTGATTTGCATCCCAGCCTGG10.8928571428571428No Hit
GGTCTAGCAGATCTCTGCCTTCTCAGGGCACCCGTGGACACAAAATAAGA10.8928571428571428No Hit
TCAGCGGAATGAGGGCATGGCCTCCTTTTGGTGAAGGACACTCCCTTGTG10.8928571428571428No Hit
GCTATGACCACTGCCCAGGCTCCCCTTCAGGCCTGATGACGGCTCACATC10.8928571428571428No Hit
GGGACCAAGTCCAAGAACCCGCCTTAACCACAGGAAGATGTTCCCTCTGC10.8928571428571428No Hit
TCTGTTAACAGACCCACAGTTTTTTCTAGAAATCAGGTTTAGTAGAGGCT10.8928571428571428No Hit
TAGGTACTGAGCTCTTAGAGCAGACCAGGATTTCCCATGAATATGAATGC10.8928571428571428No Hit
AGCTAGGACAGGGCTGTGGGAGTTAGGATGAGATCAGGCCGTGCTGGGGG10.8928571428571428No Hit
AATGTATATCTTATCACTCTTGTAAGACTTCCTTCAAGGTCTGAAAGAAA10.8928571428571428No Hit
TCTCAAAGGAGGAGGCTCAGCGGGTCCGTCTTCAAGCACAGGGGAGCCCA10.8928571428571428No Hit
CCTCATTACAGTACAACCACAAGTGCCCCCTAGCACCTCTCTCAGGAACA10.8928571428571428No Hit
CCATTTAATATATTAAATACATATTACTTTATCAAAAATCTTCATATATT10.8928571428571428No Hit
GTCTTAGGTGTTTATGCAAGCTGCTATCTAATTTTCTTTCTTACCCATAC10.8928571428571428No Hit
CTTTGAGCTATCCAAGGCTGGGAAAACAACCTGTATCAGCGTGGGTGTGG10.8928571428571428No Hit
GTTCTACGCCGCCTCAGCAAGTTTGAGGAGCCCCTCCGGACGCACTGCAG10.8928571428571428No Hit
GAACCCTTCCAGACCGAAAGGACTAGAATGAGGCTGGAGACGGCAGGCGC10.8928571428571428No Hit
GTCTATTTAGTGCAACACCATCTGTAGTTTTGTTTTCTTTTGGTGATTTC10.8928571428571428No Hit
CCTGCTGCGCAGAGAACCCTATGCGGGGCTTGATCCCAGGACCCTGGGAT10.8928571428571428No Hit
TCTTCAGTGAGTCAGGAGTGGCTCCTCAAACTTGCTTAGGCTTCGTAGAA10.8928571428571428No Hit
GGGAGAAGCAGGCTCTCCACAGAGCAGTGACTCCAACGTGGGGCTCGATC10.8928571428571428No Hit
CAGGAGCAGGAGGCCCCCCGGAGACAGGCTGGACCTTGGGCCCAGAGTTG10.8928571428571428No Hit
GACTCCAACGTGGGGCTCGATCCCAGGACCCTGGGATTATGACCTGAGCT10.8928571428571428No Hit
AGTCCCCTGTCCACCGGAAAGCCAGAGCAAAAGGGCAGGCCATCCCTACC10.8928571428571428No Hit
TCCTCCTTCTCTGCTAGCTGGTTTGGCCCCAAATTATTACGGGCGTATTT10.8928571428571428No Hit
CTGAGCAGAGAACCCTATGCGGGGCTTGATCCCAGGACCCTGGGATCATG10.8928571428571428No Hit
CCATCAAGGTTATGGATCTTCCCCGGCCCCAAAGGGCACCCGGCTACTGT10.8928571428571428No Hit
GAAGAAGCCATTTACCTATTTGATTTTCTTTTTTTGAAGAGAATCTTTTT10.8928571428571428No Hit
GTTCACATCCTGGGTTCGTCCCGCCACGCCAAAATAAGCAGACACAGCCT10.8928571428571428No Hit
GAGCACCTGTCTCTCCCCACGGTGCCGACCCCGCGCGCCCGCGCCCCCCC10.8928571428571428No Hit
GTCTTTGAGTCAATTTTGTTCCACTTTTATTAAATCTCAAAACTTGCAAG10.8928571428571428No Hit
GAAGTAGAGTGACCAGGTTCCTGCCACATGAGATCCCTGTCCTGCAGGTA10.8928571428571428No Hit
CCCTGCTCAGCAGGGAGTCTGCTTCTCCCTCTCTCTCTGCCTCTCTACCT10.8928571428571428No Hit
GAACTGCTTAGGCCCCATGCCACTTGATCAAGGCTTTCCTGGAAGCTGCC10.8928571428571428No Hit
TCCCAAACCCCGCCTGTCACCCTATTACTCCTAAATTTTTAGAGATAGTT10.8928571428571428No Hit
AGGGAGAAGCCGGCTCACCACCACGCAGGGAGCCCTACTCAGGGCTTGAT10.8928571428571428No Hit
ATGAATAACTGAATAATAATCATGCGCCATATCTTATTGAGAGAAGAGTT10.8928571428571428No Hit
CTCCTTTCCAGCCTTGATAGCTGCTCCCTCCCTGCGAGTCAGCAGGGAGC10.8928571428571428No Hit
ATGAATAAATGTCTTTATCTGCCCCCCTAAAATGCCCAGGGAGTGAGTGC10.8928571428571428No Hit
GTTTTGCACTTTGAAAAATTGTGGGTAGGCATAAGCAGGTAAGGCCCCTG10.8928571428571428No Hit
GTTTCAAAAGACAGTATGGTAGGGTTTCTTGTCTTGTTTTTTGTTTGTTT10.8928571428571428No Hit
CCTCCACCCCTGCGGAGCTCAGCTTCCGGGTGTGACTAAACAAAGAAGCC10.8928571428571428No Hit
GAACAGAGAAACAGCCGAGATAAGTGGAGCCTCAGCTATGTTGTTCTATA10.8928571428571428No Hit
GAGATTATTGCTACCATTTTGTTATGACTAAGTGTTTCCTTTTTCTATGC10.8928571428571428No Hit
ATGCAGATCTGCTCCCAGAGTGGCAGTCCTTCGCCTCTCCACCTTTCTAA10.8928571428571428No Hit
GTCTTTTGTTCTTAGGTCCCAGCCCTTAGGAGGGGATGGATGTCTTCTGA10.8928571428571428No Hit
ACACAATACCACGTTCTACAAGAAATGGGGGAGCAGGCAGCCACTGGTGG10.8928571428571428No Hit
GTGCAGAGTAAGAGGTTTCTCTGAGAAGCCCACTGAAGGTTGAATTCAAT10.8928571428571428No Hit
TCTGGATGCTATGGAGACCGAGGAAGGTGAGGGCAAGGGAAGGACGCAGG10.8928571428571428No Hit
GTTTGGGAGGGGGAGTGCTTCTCATTCGCTCTGCCTAGAGAGGTTGCCTG10.8928571428571428No Hit
CCCCAGGTTCCCGGGAGGTTTCAGTTTCTTACAATCAAAGAACTTTGGGT10.8928571428571428No Hit
GAATTTTGGAAGGGAAGTGGGTGGATCTGTGGCCAGCTCTATGCTTTTAC10.8928571428571428No Hit
GGCCATCACTAGTGTCTCAGTCACCTCTGATCTCTAGTTGGGCACACAGC10.8928571428571428No Hit
ATTTGACATATAGAAAAGCACTTAGGGAAAATCAGTGTGTGCCTAACTGA10.8928571428571428No Hit
GCAGTAGTCTGGCAAGTCCAGGGTTGGTCCCTTACTCGACCCTTCGAACA10.8928571428571428No Hit
GTTCTACGACGCCTAAGCAAGTTTGAGGAGCCACTCCTGACTCCCTGCAG10.8928571428571428No Hit
GCACGGTCAGGTAAATATTACAGCAGTCAAAGGTGCGGCTTTGGTGGGGA10.8928571428571428No Hit
GTCTGAGGGTGATGTTTCCTTTGTGATCTTCTGTCTGGATGGGGGATCAT10.8928571428571428No Hit
GTATAGTACCCTTCCCGGCAATTTGTGTAACTCCCATAGCCTATACTAAT10.8928571428571428No Hit
ATGTTGAAGCTGTGCACATACGAAAAGTCCAGGATGAAACCAAAACCCTC10.8928571428571428No Hit
CACATGGGGCAAGCCGGGTCTCGCTGAGGGACTGCTTGGATATTTTGCCC10.8928571428571428No Hit
GAGGAATGGAGTCAGCCCGTGAGGTGGGGGGATTGCACTCTGGGAAAGAT10.8928571428571428No Hit
GGGTGAGTGTGTCCCGCCACAGTGAACAGGGTCTATGCCTTACATCGCTG10.8928571428571428No Hit
ACACACCTGGCGGCGCGGCCGAAAGCAGCCATTGTCTGCAGCCCAACTAC10.8928571428571428No Hit
AAAGCAGGCTGGCCCTGGCTTAACCACAGGGAGACACAGAGATCTGTCAT10.8928571428571428No Hit
GCTCAAGGTAGGCGGGATCAAGGAGTTTGAACAGAATATCTAGGTCCATG10.8928571428571428No Hit
GGGTAACGCGCGGGTGGGTCCTGCGGTCTGGGCCCTGCGGGTGGCAGGTG10.8928571428571428No Hit
GGTCCAGGTAGCACTTTCTACTCAGACAAGAATCTGTGGTCCACCCAGGC10.8928571428571428No Hit
AACTCTCGGGGTAGGGATGGCCTGCCCTTTTGCTCTGGCGGTCCGGTGGA10.8928571428571428No Hit
ACCCAGAGATCATGACCCGAGCCAAAAACAAGAGTCCTATTCTTAATCAA10.8928571428571428No Hit
GACCAAAGAGTACCAACAAATCAAGAAGACTTGAAAGATTTTTTTATTAC10.8928571428571428No Hit
ATCGGGAAGGGCCTGCCGGGGGCTGGGGGCGGGGTGGGGGAGGCTGCAGG10.8928571428571428No Hit
TTCCCATGCTGCTTTGCTGCGTTCTTGGCGGAACCCGCTAAAGTGACTTC10.8928571428571428No Hit
TGCTATGCTTTCACAGAGCTTTTCAGCTTGGCGCGAGCCGCACATCGCTG10.8928571428571428No Hit
GATATAGGAGACAAAGGGAAACTGGCTTAGCCCCAAATTGTGGAGACCCC10.8928571428571428No Hit
GTATGGTAGGGTTTCTTGTCTTGTTTTTTGTTTGTTTTTAAGTAAACTCG10.8928571428571428No Hit
CCTTTAAGGGGGGTGGTTTATCTAAGGACCTTCCTGAGCTTGGGGGGTTT10.8928571428571428No Hit
TTAAAAACCTTATCCTAGGGCCGCCTGGGTGGCTCAGTGGGTTGGGCCGC10.8928571428571428No Hit
ATCCATTTCCATTTGTGCCACCCGCTCATTCAGTAACGTTTGTCAAGTTG10.8928571428571428No Hit
GCGCTGCACAGAGATCATAGTGAGGACTGTGGATTTCCTGCAACCCAGCA10.8928571428571428No Hit
AATGTGTTCTTTCCATTCTGGTTAGAAAGGAGGGTCAGGAGGAATTTTAG10.8928571428571428No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers