FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1966_lungLL_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1966_lungLL_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTTAGGAGGACTACGTGGCAACAGGTACGGATTTAGTGTTTCTTGAC30.1935483870967742No Hit
GTAGAGGGATGACCTCATGGTCCAGGTGACACACCTGCAGGGAGGAGACA30.1935483870967742No Hit
TTCCAGGACCTCCGCAAGCCCTGCTCTGGAAGTCAGGAGACCCCCCTGCC30.1935483870967742No Hit
GCTGAGGACCATGAGGGGGATTTAAAAGAGAATGGTAAGGTCAGGTTCGC20.12903225806451613No Hit
AGACGGGAGCGTGTCTACAGCATTTTCCGTTCTTTCCAGGACCTCCGCAA20.12903225806451613No Hit
GGTTGGAGATGTTGAAGGAGTTCTTGGGGAGCCCCCGGTCTGACAGCCTG20.12903225806451613No Hit
GTCTGTCTGTCTCTCTCCCCAGCTCTGTGTCAAATAAATAAATGGCATCT20.12903225806451613No Hit
ATCCTGTTCCTAGAAGAGGGATGTTAGGAGACATGAGCCATAGGGTCCTA20.12903225806451613No Hit
GTACCCAGCAAGTAAGAAAATAACATGGACTTGAGGGTCGGGACCCCCAG20.12903225806451613No Hit
GATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG20.12903225806451613No Hit
CGTGTCTACAGCATTTTCCGTTCTTTCCAGGACCTCCGCAAGCCCTGCTC20.12903225806451613No Hit
GGAGAGACCTGTCAAAGCATCTCTTGTCGGCCTGTACCCAGCAAGTAAGA20.12903225806451613No Hit
GGGTTGCACAGTCAGTTAAGCAGCTGACTCTTGATTTTGGCTCAAATCAA20.12903225806451613No Hit
GTAGCATTTGGTTTGCAGATACTTGGGTGAGGATTTTTATGTTTATGTTC20.12903225806451613No Hit
ACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAGTCCTTC20.12903225806451613No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG20.12903225806451613No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC20.12903225806451613No Hit
GGCCTGGGTCTTGGTCGCCTTGTCAGCTGGCCTTGGGCCACTGAGGCCAA20.12903225806451613No Hit
TCCAGGGACAGTCCTGCCCTATGGCCCGGGCTGCTGGAGTGCCCGTGCTC20.12903225806451613No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA20.12903225806451613No Hit
GAATAAATCCAACTTTCACCTGTTTTCCTAAATGGCAGGTCCCTGAAGAC20.12903225806451613No Hit
GTAAGTGGGGAGACCAAGCACTTGAGAGGCTAAGGGTCTTCTCCAGCAAG20.12903225806451613No Hit
AACCTAAGAGAAGCAATCAGCACCAATACCATAGGGAACTTTAATACCTC20.12903225806451613No Hit
CCCCAGGCCCTGCAGGTCAGCCTGCAGTGTCCCGGCACGGACCTGTGTGT20.12903225806451613No Hit
GTATTTACTGGGTTCTTGTCATGCGCTAGGTAACATTCTAGGTGCTGGGG20.12903225806451613No Hit
ATATTGGTGCTGATTGCTTCTCTTAGGTTTGTTCATATATGCTCTACATA20.12903225806451613No Hit
CAAATAAGCAACCTAACTTGATACCTCAAGGAAACAGAAAAGAACAAATA20.12903225806451613No Hit
CTTCCTACCCAGCTGTCCTGGCAAAGGGGTGGGCTGTCGAAATAAAAGCT20.12903225806451613No Hit
CTGGCCTTCACCCTCTCTGGGCCATAGCCCTGGCCTCTGAGGTGGATGTC20.12903225806451613No Hit
CGTGAAAAGGTATGCTGGCAGCTCCTGTACCGCCCAGGATGCAGAGAGGG20.12903225806451613No Hit
GTGTTACATGTCCCCAACCCCTCACCCTGTGAGGCGTGTGTCCCTGAGAC20.12903225806451613No Hit
CTCCTGATCTTGTGGTTTTGAGTTTCAACCCCACACTGGGCATAGAGCTC20.12903225806451613No Hit
CCTCATACAGTTAGTTCCCTTAAGCATCTTGCAAGATGTAAGACACAGCC20.12903225806451613No Hit
CATATGCACCAGACATGCACTCACCAGTGCTGAGTAGCTCCAGGCTGAGA20.12903225806451613No Hit
GCCTAGGATGGCACTCTGCTCACAATGCCATCTCTCGGCGTAGGGATGGC20.12903225806451613No Hit
TCGCTGTATGGCTTCCCACCTCCGTTGCCAGATGGACACAAAGGCACACA20.12903225806451613No Hit
AGTGCTCGTCCTTGGGCTTTCTGCGGGTACACCAGCATCAGCCGCACTGG20.12903225806451613No Hit
GTCATTGGAGAAGAATTTATTGTGTTTATTTACAGAGGAAAAAAAAAAGA20.12903225806451613No Hit
GTGTTGGCGTCAGGGGTGCACTCCCCTGCAGCTGGGGAGCCCCCGGGTTC20.12903225806451613No Hit
CAAGGAGGGGGAACACATGGAACCCTGGAAAACAGAGCAAGAAGATCCCA20.12903225806451613No Hit
GTCTACTAATGACCAGTAGAGAGCTTAAATAAGAAATCAAAAACTTCCAA20.12903225806451613No Hit
GCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC20.12903225806451613No Hit
GTAAATAAACACAATAAATTCTTCTCCAATGACCATATCCTGACTCCAAT20.12903225806451613No Hit
GGCCTGAGGTGGATCAACCTGGATAACAACCGGATTCGCAAGATAGACCA20.12903225806451613No Hit
CTCCTAACATCCCTCTTCTAGGAACAGGATGATTTTCTCACGCTGTAAGT20.12903225806451613No Hit
GTCTAAAACCTGGTGCTAGGTCACAGAGCTTGTCACAAGAGAGGCAGGAT20.12903225806451613No Hit
ATACTAAACACCTGGAAACAGGGGAATACTAAGCTGGTTGGCAAATCCTC20.12903225806451613No Hit
CCCTTCGACAGCGCGTCACCCCCCAGCCACGGGGGCAAACGGAATCCCCA20.12903225806451613No Hit
TGTTGAAGGAGTTCTTGGGGAGCCCCCGGTCTGACAGCCTGTTGTAGTTC20.12903225806451613No Hit
ACCATGGGGTCCTGGACTTTTGCTTGTTGGAAGTTTTTGATTTCTTATTT20.12903225806451613No Hit
TTACTAGCCTGCTGGACCTCTGGAGTTAGAGGCCCTGTCTTGGCACAAGA20.12903225806451613No Hit
GTTCCTAGAAGAGGGATGTTAGGAGACATGAGCCATAGGGTCCTAAGCGG20.12903225806451613No Hit
CCACAAGACAAGTCAATAAATTCCAGAAGCCTAGAATTTTCCCAAATGAG20.12903225806451613No Hit
GGCAAGGGCAGTGAGAGAACGCAGGAGAGGGATCCTTTCCTCCCTGCAGC20.12903225806451613No Hit
GAATGCCACCGGCCTGAGGTGGATCAACCTGGATAACAACCGGATTCGCA20.12903225806451613No Hit
GGGAAAGATCCATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATTCAGT20.12903225806451613No Hit
GATCCTGCGTCTCCCGTTGTCCACCAAACACCCCGAATTCAAGGAGATCC20.12903225806451613No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT20.12903225806451613No Hit
GGTCTGTTAGGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGG20.12903225806451613No Hit
ATACATGGCAGCCAGAGTTCAGAAGCTTACGAATAGTGCTGAGGTCAGGC20.12903225806451613No Hit
GCACAGGGAGCCCCTTTCTCCCTCTTCTACTGCCTGCTGCTCCTTCCACT20.12903225806451613No Hit
ACATAAAGACAGGACCGCTGAGGGTCATCAGCCGTGTCTCCATGGCTACG20.12903225806451613No Hit
CTACTGTGACAGCCGCAACCTCCGGAAGGTCCCTGTCATCCCCCCACGCA20.12903225806451613No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA20.12903225806451613No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)