FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1966-lungUR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1966-lungUR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680608
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA701.6954235E-551.7856336
GCGAAAG2900.039.999942
AGCGAAA3250.035.700121
CGAAAGC3250.035.6922533
GCATAGT1358.0697204E-426.857731
ACGACTG1409.991392E-425.8928175
GTTACGT1450.001224477125.0054741
GTATAAA5150.023.9372791
TATACAT4103.8198777E-1122.987775
GTTGACA5052.910383E-1120.103411
TACGTGT1850.00504529519.5945663
GTATTAT6850.019.0552651
TATTATC3501.997194E-618.642832
TTAGATC1950.006838424618.5897163
TTACGTG1950.006838424618.5897162
TACTATT3151.6822629E-518.412672
GATATAT3552.2824297E-618.3843061
TGTACAG3552.2872446E-618.3802555
GTATATG2400.001057682218.1289671
GTATAAG2801.4228461E-418.1289671
Produced by FastQC (version 0.11.5)